MRI: Development of ion-mobility enhanced electron-transfer dissociation for elucidating biological networks using a systems biology approach

MRI：离子淌度增强电子转移解离的发展，用于使用系统生物学方法阐明生物网络

• 批准号: 0923536
• 负责人: Leroy Hood
• 金额: $ 154.46万
• 依托单位: Institute for Systems Biology
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0923536&HistoricalAwards=false
• 关键词: MRI; Development; ion; mobility; enhanced

Post-translational modifications of proteins play a key role in the regulation of physiology and development. For example, reversible phosphorylation is the most common mechanism for cellular regulation in eukaryotic systems where it is estimated that roughly one third of the total protein complement of a cell is involved in this process. Coupled to this, the dynamic interactions of protein-protein and protein-DNA interactions provide a complex series of transient interactions crucial to life. In order to identify and measure all of these events in a systems biology approach, new mass spectrometry equipment and techniques must be developed. The development of high-Field Asymmetric waveform Ion Mobility Spectrometry to exploit the full potential of Electron Transfer Dissociation of peptides will provide steps towards more extensive measurement of these proteins. This project will not only enhance the research infrastructure at ISB but also integrate both educational activities and research goals to enhance the teaching, training and learning by providing innovative tools to maintain the cutting edge curriculum of courses held by ISB. Used by experimentalists and computational biologists, the data collected will ultimately inform researchers of proteome dynamics that are responsible for an organism's form and functions. Information derived from this development will be publicly accessible through the Seattle Proteome website (http://www.proteomecenter.org) as well as regular research publications.

蛋白质的翻译后修饰在生理和发育调节中起着关键作用。例如，可逆磷酸化是真核系统中最常见的细胞调控机制，据估计，大约三分之一的细胞总蛋白质补体参与了这一过程。此外，蛋白质-蛋白质和蛋白质-DNA相互作用的动态相互作用提供了对生命至关重要的一系列复杂的瞬时相互作用。为了用系统生物学的方法识别和测量所有这些事件，必须开发新的质谱学设备和技术。高场非对称波形离子迁移率光谱技术的发展，充分挖掘了多肽电子转移解离的潜力，将为更广泛地测量这些蛋白质提供新的途径。该项目不仅将加强ISB的研究基础设施，还将整合教育活动和研究目标，通过提供创新工具来保持ISB举办的课程的尖端课程，从而加强教学、培训和学习。由实验学家和计算生物学家使用，收集的数据最终将告诉研究人员蛋白质组动力学，这些蛋白质组动力学负责有机体的形式和功能。从这一发展中获得的信息将通过西雅图蛋白质组网站(http://www.proteomecenter.org))以及常规研究出版物公开获取。