Microbial Genome Sequencing: The Cenarchaeum Symbiosum Genome Project: Genome Sequence from a Psychrophilic Archaeon, the Ocean's Single-most Abundant Microbes

Edward F.蒙特利湾水族馆研究所的德隆获得了一笔赠款，用于对海洋微生物共生菌的基因组进行测序。 这种微生物属于一个不同的微生物谱系，称为微生物，是海洋中最丰富的微生物群体之一。 然而，尽管它们的丰度很高，但这些微生物从未被培养过，因此它们的生物学特性和生态学意义仍然未知。 基因组测序技术现在可以解决这个问题，甚至可以对未培养的微生物进行遗传表征。 在这个项目中，现代基因组测序策略将被开发和应用于确定和分析Cenarchaeum symbiosum的全基因组序列。这个项目将是第一个确定来自未培养的，未驯化的微生物的全基因组序列的项目之一。 所开发的方法和技术将广泛应用于许多其他研究。 开发的技术将使研究自然发生的微生物在环境中的许多不同的情况。 由于其进化的起源，Cenarchaeumsymbiosum基因组序列将提供新的见解，在微生物的热适应机制。 这一信息将是有用的研究，旨在修改或分离活性，热稳定性酶用于工业，生物技术，或商业产品。 此外，Cenarchaeum symbiosum属于一组代表海洋中最丰富的细胞类型的微生物。 这种微生物的基因组序列也应该为这些广泛而丰富的海洋微生物的功能提供重要线索。这些数据将提供给整个科学界，为不断增长的微生物全基因组序列数据库增添内容。 这些数据还将为未来的海洋生态研究提供一个起点。 这是一个微生物基因组测序奖，通过国家科学基金会和农业部之间的合作计划资助。

Edward F. DeLong of the Monterey Bay Aquarium Research Institute has been awarded a grant to sequence the genome of the marine microbe, Cenarchaeum symbiosum. This microbe belongs to a divergent lineage of microorganisms known as Archaea, and is a member of one of the most abundant microbial groups in the ocean. Despite their high abundance however, these microbes have never been cultivated, so their biological properties and ecological significance remain unknown. Genome sequencing technology can now address this problem, allowing genetic characterization of even uncultivated microbes. In this project, contemporary genome sequencing strategies will be developed and applied to determine and analyze the whole genome sequence of Cenarchaeum symbiosum.This project will be among the first to determine a whole genome sequence from an uncultivated, undomesticated microbe. The methods and techniques developed will have broad applications for many other studies. The technologies developed will enable the study of naturally occurring microorganisms in the environment in many different contexts. Due to its evolutionary origin, the Cenarchaeum symbiosum genome sequence will provide new insight into the mechanisms of thermal adaptation in microorganisms. This information will be useful for studies aiming to modify or isolate active, thermostable enzymes used in industry, biotechnology, or commercial products. Additionally, Cenarchaeum symbiosum belongs to a group of microbes that represent the most abundant cell types in the ocean. The genome sequence of this microbe should also yield important clues about the function of these widespread and abundant oceanic microorganisms. The data will be made available to the entire scientific community, adding to the growing database of whole microbial genome sequences. The data will also provide a starting point for future studies of ocean ecology. This is a Microbial Genome Sequencing Award funded through a collaborative program between the National Science Foundation and the Department of Agriculture.