CAREER: Challenging the Structure-Function Relationship of Nitrifiers and Nitrification in Activated Sludge Using Molecular Biology and Mechanistic Modeling Tools

职业：利用分子生物学和机理建模工具挑战活性污泥中硝化剂和硝化作用的结构功能关系

• 批准号: 0238858
• 负责人: Daniel Oerther
• 金额: $ 40万
• 依托单位: University of Cincinnati Main Campus
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-02-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0238858&HistoricalAwards=false
• 关键词: CAREER; Challenging; Structure; Function; Relationship

0238858 Oerther The primary research goal of this NSF CAREER proposal is to reduce required sludge ages for successful nitrification in activated sludge systems. The primary educational goals are to integrate modeling of biological treatment systems with existing molecular biology courses taught at the University of Cincinnati and to conduct a preconference workshop on molecular biology and modeling tools at the Water Environment Federation Technical Exposition and Conference (WEFTEC). This research and education plan expands the ongoing efforts of the Principal Investigator (PI) to develop and demonstrate novel approaches to bridge the gap between engineers and ecologists by challenging the hypothesis that, increased diversity among nitrifying microorganisms reduces required sludge ages for nitrification in suspended growth activated sludge systems with mechanistic models and to transfer these technologies to practitioners.Intellectual Merit. Successfully bridging the gap between function-based engineering and structure-based microbiology represents a fundamental crosscutting challenge that must be addressed before the results of molecular biology tools can be fully integrated with environmental engineering practice. Based upon his interdisciplinary education, including degrees in the biological sciences (B.A.) as well as environmental engineering (B.S., M.S., Ph.D.), the PI is uniquely qualified to meet this challenge. The PI's multidisciplinary approach to the optimization of nitrification and the integration of state-of-the-art research techniques into the undergraduate and graduate curricula fit with the institutional goals of the University of Cincinnati and the five-year plan of the Department of Civil and Environmental Engineering.Broader Impacts. The PI has a strong history of supporting women and minority researchers in his laboratory through the Women In Science and Engineering (WISE) program, Rowe Center for Women in Engineering of the College of Engineering at UC. Four of his current seven students are women and three are minority. The CLSM described in the proposal will dramatically enhance the research and teaching infrastructure of UC by providing the first CLSM facility on the main (non-medical) campus. The proposed WEFTEC pre-conference workshop represents a first effort to transfer molecular biology tools from academia to environmental engineering practitioners.

0238858其他NSF CAREER提案的主要研究目标是降低活性污泥系统中成功硝化所需的污泥年龄。主要的教育目标是将生物处理系统的建模与辛辛那提大学现有的分子生物学课程结合起来，并在水环境联合会技术博览会和会议（WEFTEC）上举办一个关于分子生物学和建模工具的会前讲习班。这项研究和教育计划扩大了首席研究员（PI）正在进行的努力，通过挑战这样的假设，即硝化微生物多样性的增加减少了悬浮生长活性污泥系统中硝化所需的污泥年龄，并将这些技术转让给实践者，从而开发和展示了弥合工程师和生态学家之间差距的新方法。知识价值。成功地弥合基于功能的工程和基于结构的微生物学之间的差距是一个基本的横切挑战，必须在分子生物学工具的结果与环境工程实践完全整合之前解决。基于他的跨学科教育，包括生物科学（学士学位）和环境工程（学士学位，硕士学位，博士学位），PI是唯一有资格迎接这一挑战的人。该项目采用多学科方法优化硝化过程，并将最先进的研究技术整合到本科和研究生课程中，这与辛辛那提大学的机构目标和土木与环境工程系的五年计划相吻合。更广泛的影响。加州大学工程学院Rowe工程女性中心在通过科学与工程女性（WISE）项目支持实验室的女性和少数族裔研究人员方面有着悠久的历史。他目前的7名学生中有4名是女性，3名是少数民族。提案中描述的CLSM将通过在主校区（非医疗）提供第一个CLSM设施，极大地增强加州大学的研究和教学基础设施。提议的WEFTEC会前研讨会是将分子生物学工具从学术界转移到环境工程实践者的第一次努力。