CAREER: Towards Synthetic Cytoplasm: Volume Exclusion and Aqueous Phase Separation in Giant Vesicles

职业：走向合成细胞质：巨囊泡中的体积排除和水相分离

• 批准号: 0239629
• 负责人: Christine Keating
• 金额: $ 51.2万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-02-15 至 2009-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0239629&HistoricalAwards=false
• 关键词: CAREER; Towards; Synthetic; Cytoplasm; Volume

This CAREER award to Professor Christine Keating of Pennsylvania State University is supported by the Analytical and Surface Chemistry Program in the Chemistry Division and by Cellular Systems in the Division of Molecular and Cellular Biosciences. In this project, the properties of aqueous two-phase systems will be studies as contained within giant unilamellar vesicles. Questions to be addressed include the partitioning of proteins and other macromolecules between the two phases, and how this partitioning is affected by encapsulation within the vesicle. The partitioning could either follow Michaelis-Menton or may show a sigmoidal, i.e., "switchlike" response curve, with the latter giving access to unique vesicle properties. One property to be examined is vesicle locomotion based on the reversible polymerization of cytoskeletal proteins within these "synthetic cells" with the driving force provided by a temperature and/or chemical gradient. The overall goal of the project is elucidation of the functional architectures used by cells, a knowledge which can then be applied to abiotic nanostructures.A comprehensive education and outreach plan is described, including outreach to high school students through a high school biology teacher. High school teachers have already worked in the PI's lab during summer months and a new teacher is to be supported for one summer with this award. These teachers have worked with state-of-the-art instrumentation and have been key in development of new experiments with biosensors and cell structure appropriate for the high school laboratory. In addition, the PI will develop a new graduate-level course on biorecognition.

宾夕法尼亚州立大学教授克莉丝汀的职业生涯奖得到了化学系分析和表面化学项目以及分子和细胞生物科学系细胞系统的支持。 在这个项目中，含水两相系统的性质将被研究为包含在巨大的单层囊泡。 需要解决的问题包括蛋白质和其他大分子在两相之间的分配，以及这种分配如何受到囊泡内封装的影响。 分区可以遵循Michaelis-Menton或可以显示S形，即，“开关样”响应曲线，后者提供了独特的囊泡特性。 待检查的一个特性是基于这些“合成细胞”内的细胞骨架蛋白的可逆聚合的囊泡运动，其驱动力由温度和/或化学梯度提供。 该项目的总体目标是阐明细胞使用的功能结构，然后可以应用于非生物nanostructure.A全面的教育和推广计划，包括通过高中生物老师推广到高中学生的知识。 高中教师已经在PI的实验室在夏季工作，一个新的教师将支持一个夏天与这个奖项。 这些教师使用了最先进的仪器，并在开发适合高中实验室的生物传感器和细胞结构的新实验方面发挥了关键作用。 此外，PI将开发一个新的生物识别研究生课程。