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The analysis of sink/source function of rice based on genome information and chromosome segment substitution lines

基于基因组信息和染色体片段替换系的水稻库/源功能分析

基本信息

批准号：
17208003
负责人：
OHSUGI Ryu
金额：
$ 30.2万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

The objectives of this research is to elucidate some genetic factors related to sink/source functions of rice based on genome information and chromosome segment substitution lines (CSSLs).(1) Thirty nine CSSLs with a chromosome segment of Kasalath in the genetic background of Koshihikari were used for clarifying differences in RubisCO content in leaf and non-structural carbohydrate (NSC) concentration in leaf sheath between CSSLs and Koshihikari. As a result, RubisCO content and NSC concentration of one CSSL (Part of chromosome 10 of Koshihikari was substituted by Kasalath counterpart) were significantly higher than those of Koshihikari.(2) Another 39 CSSLs with a chromosome segment of Habataki in the genetic background of Sasanishiki were used for clarifying differences in RubisCO content in leaf and non-structural carbohydrate (NSC) content in leaf sheath between CSSLs and Sasanishiki. As a result, RubisCO and NSC content of one CSSL (a part of chromosome 5 of Sasanishiki was substit … More uted by Habataki counterpart) were significantly higher than those of Sasanishiki.(3) A cross between SL417 (CSSL line containing NSC allele in the experiment (2)) and Sasanishiki were made, and BC1F4 population containing 5000 plants for QTL fine mapping were generated. The QTL qNSC5-1 was successfully delimited to an approximately 60kb region, which defined by two STS markers on the BAC clone. This region contains seven open reading frames (ORFs) consisting of a carbohydrate metabolism enzyme and so on.(4) A simple, low-cost capillary electrophoresis/mass spectrometry (CE/MS) method was newly established for the simultaneous analysis of amino acids and small carboxylic acids (glycerate, lactate, fumarate, succinate, malate, tartrate, citrate, iso-citrate, cis-aconitate and shikimate). All CE/MS experiments were performed using a single uncoated fused-silica capillary and with a single separation electrolyte, formic acid. By changing the polarity of the MS during CE separation, both amino acids and small carboxylic acids were detectable in a single electrophoresis analysis run Less

本研究的目的是基于基因组信息和染色体片段置换系(CSSL)来阐明与水稻库/源功能相关的一些遗传因素。(1)以39个染色体片段为Kasalath的Koshihikari代换系为材料，阐明了CSSL和Koshihikari在叶片Rubisco含量和叶鞘中非结构性碳水化合物(NSC)浓度上的差异。结果表明，1个CSSL(Koshihikari 10号染色体的一部分被Kasalath对应体取代)的Rubisco含量和NSC含量显著高于Koshihikari。(2)利用另外39个Sasanishiki遗传背景中含有Habataki染色体片段的CSSL，分析了CSSL和Sasanishiki在叶片Rubisco含量和叶鞘非结构性碳水化合物(NSC)含量上的差异。结果表明，1个CSSL(Sasanishiki 5号染色体的一部分)的Rubisco和Nsc含量取代了…(3)以SL417(试验2中含有NSC等位基因的CSSL系)与Sasanishiki杂交，构建了包含5000株QTL精细定位的BC1F4群体。成功地将QTL qNSC5-1定位在一个约60kb的区域，该区域由BAC克隆上的两个STS标记决定。该区域包含7个开放阅读框(ORF)，由碳水化合物代谢酶等组成。(4)建立了一种简单、低成本的毛细管电泳质谱(CE/MS)同时分析氨基酸和小羧酸(甘油酸、乳酸、富马酸、琥珀酸、苹果酸、酒石酸、柠檬酸、异柠檬酸、顺式乌头酸和莽草酸)的方法。所有的CE/MS实验都是使用单一的未涂层熔融石英毛细管和单一的分离电解质甲酸。通过在CE分离过程中改变MS的极性，在一次电泳分析中可以检测到更少的氨基酸和小羧酸