Microbial Genome Sequencing: Genome Sequencing of the Protistan Parasite Perkinsus Marinus

微生物基因组测序：原生生物寄生虫 Perkinsus Marinus 的基因组测序

• 批准号: 0333240
• 负责人: Najib El-Sayed
• 金额: $ 165.65万
• 依托单位: Institute for Genomic Research
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-15 至 2006-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0333240&HistoricalAwards=false
• 关键词: Microbial; Genome; Sequencing; Protistan; Parasite

Perkinsus marinus is a facultative intracellular parasite that causes "Dermo" disease in the eastern oyster Crassostrea virginica. In recent decades it has devastated oyster beds along the Gulf of Mexico and the Atlantic coast, with catastrophic consequences for local fisheries and the health of coastal waters. Additional Perkinsus species threaten shellfisheries of clams and abalone worldwide. Research on P. marinus has resulted in management strategies to minimize the effects of the disease on oyster harvests but the distribution of the parasite continues to spread and no effective treatment for Dermo disease has been developed. In order to facilitate further research on this ecologically and economically important parasite, the complete sequence of the 28 Mb P. marinus genome will be determined by The Institute for Genomic Research (TIGR) and the Center for Marine Biotechnology (COMB). In addition, 10,000 Expressed Sequence Tags (ESTs) will be generated from cDNA libraries and used to produce a P. marinus Gene Index. Sequence data and annotation will be made available to the scientific community via P. marinus Genome and Gene Index web sites at TIGR, as well as by release to GenBank. The project will provide significant educational and training activities including support for postdoctoral trainees, summer students, and interns. The research activities will also be used to develop the Perkinsus/oyster disease model for conceptual and hands-on experiences within the COMB SciTech program for K-12 students from the Baltimore region.

Perkinsus marinus是一种兼性细胞内寄生虫，可引起东方牡蛎的“皮肤病”。近几十年来，它破坏了墨西哥湾和大西洋沿岸沿着的牡蛎养殖场，对当地渔业和沿海沃茨的健康造成了灾难性的后果。其他Perkinsus物种威胁世界各地的蛤和鲍鱼贝类渔业。对P. marinus的研究导致了管理策略，以尽量减少疾病对牡蛎收成的影响，但寄生虫的分布继续蔓延，没有有效的治疗皮肤病已经开发出来。为了促进对这种具有生态和经济重要性的寄生虫的进一步研究，28 Mb P. marinus基因组的完整序列将由基因组研究所（TIGR）和海洋生物技术中心（COMB）确定。此外，将从cDNA文库中产生10，000个表达序列标签（EST），并用于产生P. marinus基因索引。序列数据和注释将通过TIGR的P. marinus Genome和Gene Index网站提供给科学界，并发布到GenBank。该项目将提供重要的教育和培训活动，包括支持博士后学员，暑期学生和实习生。研究活动还将用于开发Perkinsus/牡蛎疾病模型，用于巴尔的摩地区K-12学生的COMB SciTech计划中的概念和实践经验。