Functional analysis of novel head patterning genes

新型头部模式基因的功能分析

• 批准号: 164522881
• 负责人: Professor Dr. Gregor Bucher
• 金额: --
• 依托单位: Abteilung Evolutionäre Entwicklungsgenetik
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/164522881?language=en
• 关键词: Functional; analysis; novel; head; patterning

Insects have evolved many different head morphologies as adaptation to different habitats and lifestyles, for instance in homed beetles, snout beetles and stalked eyed flies. The developmental basis of this diversity is not understood. As prerequisite to understand these mechanisms, we want to elucidate the principles of typical head formation in the embryo of the red flour beetle Tribolium castaneum. We focus on an anterior median region (AMT) which harbors mouth and upper lip (labrum). Our preliminary data indicates that the AMT is patterned independently from surrounding tissues which could explain the diversity of the respective structures of the head cuticle. A comprehensive set of genes involved in AMT formation will be identified in the iBeetle-Screen. We will determine their expression and investigate their function. Moreover, we want to understand the genetic interactions of these genes in order to elucidate the AMT gene regulatory network. The analysis of cell division, migration and cell death will allow understanding the generation of the cuticle phenotypes. So far, the AMT is defined by expression patterns in embryos only while its borders in the larval or adult head are now known. By marking cells with fluorescent markers we will follow their destiny throughout development. We will test the genes involved in embryonic AMT patterning also for a function during metamorphosis and identify from the screen all genes required for AMT patterning during metamorphosis. The comparison will allow judging whether the gene sets are highly overlapping or rather distinct.

昆虫进化出了许多不同的头部形态，以适应不同的栖息地和生活方式，例如，在归巢甲虫、鼻子甲虫和有柄眼睛的苍蝇身上。这种多样性的发展基础尚不清楚。作为了解这些机制的前提，我们想要阐明红粉甲虫胚胎中典型头部形成的原理。我们将重点放在前正中区域(AMT)，该区域包含口腔和上唇(唇骨)。我们的初步数据表明，AMT是独立于周围组织形成的，这可以解释头部角质层各自结构的多样性。IBeetle-Screen将识别一组与AMT形成有关的全面基因。我们将确定它们的表达并研究它们的功能。此外，我们还希望了解这些基因之间的遗传相互作用，以阐明AMT基因的调控网络。对细胞分裂、迁移和细胞死亡的分析将有助于理解角质层表型的产生。到目前为止，AMT只由胚胎中的表达模式定义，而它在幼虫或成虫头部的边界现在是已知的。通过用荧光标记标记细胞，我们将在整个发育过程中跟随它们的命运。我们将测试参与胚胎AMT模式形成的基因在变态过程中的功能，并从屏幕上识别变态过程中AMT模式所需的所有基因。通过比较，可以判断这些基因组是高度重叠还是截然不同。