SGER: Charge Amplifier for Biological Mass Spectrometry

SGER：用于生物质谱分析的电荷放大器

• 批准号: 0353028
• 负责人: John Price
• 金额: $ 6.47万
• 依托单位: University of Colorado at Boulder
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-11-01 至 2004-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0353028&HistoricalAwards=false
• 关键词: SGER; Charge; Amplifier; Biological; Mass

A grant has been awarded to John C. Price for the development of a cryogenic parametric charge amplifier with improved sensitivity for biological mass spectrometry applications. A current major trend in biological mass spectrometry is extension to the megadalton mass range through the use of electrospray and laser desorption ionization techniques. Many studies of importance to biotechnology,medical diagnosis, and molecular biology are possible in this mass range,including the identification of intact viruses and study of protein/nucleic acid complexes. Soft ionization techniques produce megadalton ions with tens to thousands of elementary charges and a broad charge distribution. Existing mass spectrometers only measure the mass-to-charge ratio,and in many situations it is not possible to resolve or identify the individual charge states,so that multiple charging places severe limits on mass resolution. This problem can addressed by charge detection mass spectrometry (CD-MS), a technique in which ions are passed through a Faraday tube connected to a sensitive charge amplifier, so that the charge may be directly measured on individual ions. However, CD-MS is limited by the poor resolution of existing charge amplifiers, not better than about 50 electrons root-mean-square (e- rms). This research project will develop a new type of cryogenic parametric charge amplifier with improved sensitivity for CD-MS applications.

一项补助金已授予约翰C。用于生物质谱应用的具有改进灵敏度的低温参量电荷放大器的开发价格。 目前生物质谱的主要趋势是通过使用电喷雾和激光解吸电离技术扩展到兆道尔顿质量范围。 在这个质量范围内，许多对生物技术、医学诊断和分子生物学具有重要意义的研究都是可能的，包括完整病毒的鉴定和蛋白质/核酸复合物的研究。软电离技术产生具有数万至数千个基本电荷和宽电荷分布的兆道尔顿离子。现有的质谱仪仅测量质荷比，并且在许多情况下不可能解析或识别单个电荷状态，使得多次充电对质量分辨率造成严重限制。这个问题可以通过电荷检测质谱（CD-MS）来解决，该技术中离子通过连接到灵敏电荷放大器的法拉第管，使得可以直接测量单个离子上的电荷。 然而，CD-MS受限于现有电荷放大器的低分辨率，不优于约50个电子均方根（e-rms）。本研究计划将发展一种新型的低温参量电荷放大器，以提高CD-MS应用的灵敏度。