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DISSERTATION RESEARCH: Evolutionary Genetics of the Lac Operon in E. coli

论文研究：大肠杆菌紫胶操纵子的进化遗传学

基本信息

批准号：
0407755
负责人：
Daniel Dykhuizen
金额：
$ 0.89万
依托单位：
SUNY at Stony Brook
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2004
资助国家：
美国
起止时间：
2004-07-01 至 2006-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0407755&HistoricalAwards=false
关键词：
DISSERTATION RESEARCH Evolutionary Genetics Lac

项目摘要

Genes code for proteins, but most do not do so at a constant rate under all conditions. These differences in the expression of genes are thought to underlie much of the diversity of organisms. This project uses the lac operon of E. coli, which is responsible for metabolizing lactose, to examine the forces affecting the evolution genetic regulation. Using E. coli collected from high and low lactose environments, the investigators will determine the differences in expression of the lac operon associated with these environmental differences. DNA sequencing of the operon will elucidate the genetic basis of these adaptive differences. The researchers will use recombinant DNA techniques to manipulate the regulation of the genes and then measure the fitness of manipulated strains in the laboratory. Studies of the lac operon have played a major role in understanding how genetic regulation occurs. This study will use the system to understand the evolution of genetic regulation, a major cause of biodiversity. Changes in genetic regulation are likely play a major part in the evolution of pathogenic bacteria and bacteria which function in basic ecological processes like decomposition. The basic knowledge gained in these studies will help our future understanding of these economic and social impacts of biodiversity.

基因编码蛋白质，但大多数并不是在所有条件下都以恒定的速度编码。基因表达的这些差异被认为是生物体多样性的基础。该项目使用负责代谢乳糖的大肠杆菌的乳胶操纵子来检测影响进化基因调控的力量。使用从高乳糖和低乳糖环境中收集的大肠杆菌，研究人员将确定与这些环境差异相关的乳胶操纵子表达的差异。操纵子的DNA测序将阐明这些适应性差异的遗传基础。研究人员将使用重组DNA技术来操纵基因的调节，然后在实验室测量操纵菌株的适合度。对乳胶操纵子的研究在理解基因调控是如何发生的方面发挥了重要作用。这项研究将使用该系统来了解基因调控的演变，这是生物多样性的一个主要原因。基因调控的变化可能在病原菌和细菌的进化中发挥重要作用，这些细菌在基本的生态过程中发挥作用，如分解。从这些研究中获得的基本知识将有助于我们今后了解生物多样性对经济和社会的影响。