DISSERTATION RESEARCH: Coevolutionary Diversification in Septobasidium, a Fungal Symbiont of Scale Insects

论文研究：介壳虫真菌共生体 Septobasidium 的共同进化多样化

• 批准号: 0408011
• 负责人: Rytas Vilgalys
• 金额: $ 1.2万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0408011&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Coevolutionary; Diversification; Septobasidium

Nontechnical AbstractThis research focuses on the biology of Septobasidium, a fungal parasiteon scale insects. Scale insects are common agricultural and horticulturalpests, and Septobasidium is a fungus that sterilizes the insects that itinfects. However, colonies of Septobasidium require both infected anduninfected insects to survive. Because Septobasidium lives in this complexassociation with its host scale insects its diversification is expected tobe tightly linked to diversity and diversification in host scale insects.This research seeks to elucidate any correlative patterns ofdiversification in the hosts and parasites and to determine the processesthat lead to the formation of those patterns. The proposed research hastwo main parts. The first part uses DNA sequence data from both fungi andhost insects to examine large-scale patterns of host association andcospeciation that structure diversity within the symbiosis. Phylogeneticmethods are used to infer the history of hosts and parasites, and thesereconstructed histories are compared to determine how host evolution isrelated to parasite evolution. The second part implements methods toanalyze DNA sequence data from multiple populations of Septobasidiumspecies from across the southeastern United States in order to identifyprocesses that generate the large-scale patterns. Population processessuch as migration and dispersal are estimated from DNA sequence variationseparately and simultaneously for both hosts and parasites. Comparingthese processes from several different fungal species with different hostinsects and ecologies but from overlapping geographic ranges willdetermine how strongly the host and parasite population processesinfluence each other. Combining the two major parts provides insight intoevolutionary diversification in this symbiosis and represents the firststudy to apply critical genetic approaches to understand the ecology andevolution of this classic fungus/ insect relationship.This study benefits the public in several specific ways. This studyimproves cross discipline communication through collaboration of amycology student and an expert entomologist. The study also providesopportunities for undergraduate students to gain research experience.Finally, the research furthers understanding of fungal/ scale insectparasitism that may lead to biological control of plant pests.

非技术摘要这项研究的重点是一种真菌寄生虫鳞片昆虫的spepobasidium的生物学。规模昆虫是常见的农业和园艺种植，spepo虫是一种对昆虫造成的昆虫进行消毒的真菌。然而，隔膜的菌落需要感染和无侵染的昆虫才能生存。由于隔叶叶层与其宿主尺度昆虫生活在这种复杂的调查中，因此有望与宿主尺度昆虫中的多样性和多样化紧密相关。这项研究旨在阐明宿主和寄生虫中的任何相关模式，并确定Processesthat导致这些模式的形成。拟议的研究HASTWO主要部分。第一部分使用来自真菌和主主昆虫的DNA序列数据来检查宿主关联和综合性的大规模模式，这些模式在共生中结构多样性。系统发育方法用于推断宿主和寄生虫的历史，并比较这些遗传学的历史，以确定宿主的进化如何与寄生虫进化相关。第二部分实现方法，从来自美国东南部的多个隔膜种群中的DNA序列数据进行to型DNA序列数据，以识别产生大规模模式的过程。宿主和寄生虫同时估算了DNA序列变异，从DNA序列变异估算了人口过程。比较来自几种不同的真菌物种与不同宿主体和生态的过程，但重叠的地理范围将确定宿主和寄生虫种群过程之间的强烈强烈。将这两个主要部分结合在一起，可以洞悉该共生中的进化多样化，并代表了采用关键遗传方法来理解这种经典真菌/昆虫关系的生态和进化的第一个研究。这项研究以多种特定的方式使公众受益。这项研究通过研究生学生和专家昆虫学家的合作来跨学科交流。这项研究还为本科生获得研究经验提供了企业，从而提高了对真菌/尺度昆虫散土症的理解，这可能会导致对植物有害生物的生物控制。