Microbial Genome Sequencing: Comparative Analysis of In vivo and In vitro Transcripts (ESTs) of Beauveria bassiana

球孢白僵菌是一种机会致病真菌，它已经进化出一系列策略，用于成功感染和降解广泛的节肢动物宿主。昆虫病原真菌能够感染活的昆虫，自巴斯德以来，科学家们已经建议将其用于控制各种农业，入侵和滋扰性害虫。除了它们在生物控制机制中的用途之外，白僵菌属是第二种最广泛使用的全细胞真核生物催化剂，并且负责一系列化学和合成方法仍然难以实现的生物转化和补救。然而，真菌昆虫致病性的分子机制在很大程度上是未知的。项目目标是隔离和注释B。表达序列标签（ESTs）。该项目的研究成果将对B的基因表达进行深入分析。为分子鉴定和基因发现提供了大量的序列数据库。这些信息将通过一个来源（NCBI）网站提供给科学界。该项目包括课程编制，以便向研究生和本科生提供生物信息学的理论和实践课程。研究使命产生的数据将用于为生物信息学和基因组学的学生提供实践学习工具。该实验室致力于积极为代表性不足的群体提供机会，并将这些目标整合到研究生，本科生和高中学生的托管中。

Beauveria bassiana is an opportunistic fungal pathogen that has evolved a spectrum of strategies for the successful infection and degradation of a broad range of arthropod hosts. Entomopathogenic fungi are able to infect living insects, and scientists since Pasteur have suggested their use for controlling a variety of agricultural, invasive, and nuisance pests. Aside from their use in biocontrol regimes, Beauveria sp are the second most widely used whole cell eukaryotic biocatalysts, and are responsible for a range of biological transformations and remediations that remain elusive to chemical and synthetic methods. Nonetheless, the molecular mechanisms of fungal entomopathogenicity are largely unknown. The project objectives are to isolate and annotate B. bassiana expressed sequence tags (ESTs). Research derived from the project will yield in depth analyses of gene expression in B. bassiana, providing a substantial sequence database for molecular characterization and gene discovery. This information will be made available to the scientific community from a source (NCBI) website. The project includes curriculum development in order to present students, at both the graduate and undergraduate levels, theoretical and practical lessons in bioinformatics. Data generated from the research mission will be used to provide a hands-on learning tool for students in bioinformatics and genomics. The laboratory is dedicated to actively providing opportunities for underrepresented groups, and has integrated these aims in hosting students at the graduate, undergraduate, and high school levels.