Microbial Genome Sequencing: Comparative Analysis of In vivo and In vitro Transcripts (ESTs) of Beauveria bassiana

微生物基因组测序：白僵菌体内和体外转录本 (EST) 的比较分析

• 批准号: 0412137
• 负责人: Nemat Keyhani
• 金额: $ 17.32万
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-10-01 至 2006-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0412137&HistoricalAwards=false
• 关键词: Microbial; Genome; Sequencing; Comparative; Analysis

Beauveria bassiana is an opportunistic fungal pathogen that has evolved a spectrum of strategies for the successful infection and degradation of a broad range of arthropod hosts. Entomopathogenic fungi are able to infect living insects, and scientists since Pasteur have suggested their use for controlling a variety of agricultural, invasive, and nuisance pests. Aside from their use in biocontrol regimes, Beauveria sp are the second most widely used whole cell eukaryotic biocatalysts, and are responsible for a range of biological transformations and remediations that remain elusive to chemical and synthetic methods. Nonetheless, the molecular mechanisms of fungal entomopathogenicity are largely unknown. The project objectives are to isolate and annotate B. bassiana expressed sequence tags (ESTs). Research derived from the project will yield in depth analyses of gene expression in B. bassiana, providing a substantial sequence database for molecular characterization and gene discovery. This information will be made available to the scientific community from a source (NCBI) website. The project includes curriculum development in order to present students, at both the graduate and undergraduate levels, theoretical and practical lessons in bioinformatics. Data generated from the research mission will be used to provide a hands-on learning tool for students in bioinformatics and genomics. The laboratory is dedicated to actively providing opportunities for underrepresented groups, and has integrated these aims in hosting students at the graduate, undergraduate, and high school levels.

球孢白僵菌是一种机会性真菌病原体，它已经进化出一系列策略来成功地感染和降解广泛的节肢动物宿主。昆虫病原真菌能够感染活的昆虫，自巴斯德以来，科学家们建议使用它们来控制各种农业、入侵和公害害虫。除了在生物防治系统中的应用外，白僵菌是第二广泛使用的全细胞真核生物催化剂，并负责一系列的生物转化和修复，这些仍然是化学和合成方法难以捉摸的。然而，真菌昆虫致病的分子机制在很大程度上是未知的。该项目的目标是分离和注释球孢杆菌表达序列标签(EST)。该项目的研究将产生对球孢杆菌基因表达的深入分析，为分子表征和基因发现提供大量的序列数据库。这些信息将从一个来源(NCBI)网站提供给科学界。该项目包括课程开发，以便向研究生和本科生提供生物信息学的理论和实践课程。研究任务产生的数据将用于为生物信息学和基因组学学生提供动手学习工具。该实验室致力于积极为代表不足的群体提供机会，并将这些目标整合到招收研究生、本科生和高中学生的工作中。