Whole Cell and Regulatory Protein-Based Sensors in Bioanalysis

肯塔基大学的Sylvia Daunert教授得到了分析和表面化学计划的支持，继续研究基于全细胞细菌的传感系统。该项目的目标有两个：第一，制造实用的全细胞传感器，足够稳定，可以储存和现场使用。这个想法是在光盘上使用微流控格式的溶血和/或孢子。其次，PI将应用整个细胞作为信号化学物质的传感器，细胞使用这些化学物质以一种称为“群体感应(QS)”的模式来“决定”抗生素的生产、毒力和生物发光的释放。对细菌信号的干预已被提议作为传统抗生素疗法的替代方案。这项提议涵盖化学信号、传感和复杂系统等领域，重点是尖端技术。学生，包括那些来自阿巴拉契亚的学生，接受了这种高度跨学科的传感方法的培训，这是一个国家在打击恐怖主义方面的需要。

Professor Sylvia Daunert of the University of Kentucky is supported by the Analytical and Surface Chemistry Program to continue work on a whole-cell bacteria-based sensing system. The project goals are two-fold: first, to make practical whole-cell sensors that are stable enough for storage and field use. The idea is to use lypohilization and/or spores in a microfluidic format on a compact-disc. Secondly, the PI will apply whole cells as sensors of signalling chemicals that cells use to "decide" things like antibiotic production, virulence and release of bioluminescence in a mode called "quorum sensing (QS)." Intervention in bacteria signalling has been proposed as an alternative to traditional antibiotic therapy. This proposal spans the areas of chemical signalling, sensing, and complex systems, with an emphasis on cutting edge technology. Students, including those from Appalachia, are trained in this highly interdisciplinary approach to sensing, an area of national need in combatting terrorism.