Whole Cell and Regulatory Protein-Based Sensors in Bioanalysis

生物分析中基于全细胞和调节蛋白的传感器

• 批准号: 0416553
• 负责人: Sylvia Daunert
• 金额: $ 36万
• 依托单位: University of Kentucky Research Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0416553&HistoricalAwards=false
• 关键词: Whole; Cell; Regulatory; Protein; Based

Professor Sylvia Daunert of the University of Kentucky is supported by the Analytical and Surface Chemistry Program to continue work on a whole-cell bacteria-based sensing system. The project goals are two-fold: first, to make practical whole-cell sensors that are stable enough for storage and field use. The idea is to use lypohilization and/or spores in a microfluidic format on a compact-disc. Secondly, the PI will apply whole cells as sensors of signalling chemicals that cells use to "decide" things like antibiotic production, virulence and release of bioluminescence in a mode called "quorum sensing (QS)." Intervention in bacteria signalling has been proposed as an alternative to traditional antibiotic therapy. This proposal spans the areas of chemical signalling, sensing, and complex systems, with an emphasis on cutting edge technology. Students, including those from Appalachia, are trained in this highly interdisciplinary approach to sensing, an area of national need in combatting terrorism.

肯塔基州大学的Sylvia Daunert教授得到了分析和表面化学项目的支持，继续研究基于全细胞细菌的传感系统。 该项目的目标有两个方面：首先，制造实用的全细胞传感器，这些传感器足够稳定，可用于存储和现场使用。 这个想法是使用冻干和/或孢子在一个紧凑的光盘上的微流体格式。其次，PI将应用整个细胞作为信号化学物质的传感器，细胞使用这些化学物质来“决定”诸如抗生素生产、毒性和生物发光的释放等事情，这种模式称为“群体感应（QS）”。“对细菌信号传导的干预已被提出作为传统抗生素治疗的替代方案。 该提案涵盖化学信号、传感和复杂系统领域，重点是尖端技术。 学生，包括来自阿巴拉契亚的学生，接受这种高度跨学科的传感方法的培训，这是国家打击恐怖主义的一个领域。