Spore-Based Biosensing Systems: A Stabilized Dormant-Active Approach to Whole-Cell Biosensors
基于孢子的生物传感系统:全细胞生物传感器的稳定休眠活性方法
基本信息
- 批准号:0718844
- 负责人:
- 金额:$ 40.5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2007
- 资助国家:美国
- 起止时间:2007-08-15 至 2011-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The Analytical and Surface Chemistry program in the Division of Chemistry will support the research program of Prof. Sylvia Daunert of the University of Kentucky. The research program of Prof. Daunert focuses on the development of whole-cell sensing systems. Whole-cell sensing systems are ideal analytical tools in biomedical analysis as well as in environmental monitoring due to their sensitivity, selectivity, and rapidity. Their potential would further be enhanced if they could be used effectively for on-site applications. In that regard, there is a need for methods that improve the shelf-life and transportability of whole-cell biosensing systems, and allow for their adaptation to miniaturized systems for field analysis. To that end, Prof. Daunert and her students will develop a method based on the use of bacterial spores for the long-term maintenance of the viability and activity of bacterial sensing cells, and integrate such preserved sensor cells into portable systems for on-site sensing. Bacterial spores were chosen as the protective component of whole-cell sensors since they are a resting form of life that exhibit long-term resistance towards extreme environmental conditions, and are able to resume full metabolic activity when properly stimulated. Specifically, Prof. Daunert and her students will develop genetically engineered whole-cell sensing systems for various target analytes using spore-forming bacteria. They will then produce spores as means of preservation, storage and transport of the sensing cells. Subsequently, they will incorporate these dormant sensors into a miniaturized compact disk-like microfluidic platform. Additionally, they will immobilize them on paper strips as a simple ready-to-use configuration. Both systems can be transported to the field, where the spores can be germinated to vegetative cells and employed for sensing. Prof. Daunert envisions that this new strategy could expand the use of whole-cell biosensors for on-site analysis, not only in mild environments, but also in environments and applications in which they could not be employed previously. These include extreme environments, such as deserts, polar regions and space, as well as the remote sensing of chemical warfare agents used for bioterrorism. The impact of this work could be strong in developing countries, where rapid, simple and inexpensive analytical tools for monitoring human health and the environment are needed, and limitations are often posed by unfavorable climate conditions and inadequate distribution and storage facilities. The project will provide excellent training opportunities to students in a cutting edge multidisciplinary research area of high societal impact.
化学部的分析和表面化学计划将支持肯塔基州大学的Sylvia Daunert教授的研究计划。Daunert教授的研究计划侧重于全细胞传感系统的开发。全细胞传感系统由于其灵敏度、选择性和快速性而成为生物医学分析以及环境监测中的理想分析工具。 如果能够有效地将其用于现场应用,其潜力将进一步增强。在这方面,需要改进全细胞生物传感系统的保质期和可运输性的方法,并允许它们适应用于现场分析的小型化系统。 为此,Daunert教授和她的学生将开发一种基于使用细菌孢子的方法,用于长期维持细菌传感细胞的活力和活性,并将这种保存的传感器细胞集成到便携式系统中进行现场传感。 选择细菌孢子作为全细胞传感器的保护性组分,因为它们是一种静息形式的生命,对极端环境条件表现出长期抵抗力,并且在适当刺激时能够恢复全部代谢活动。 具体来说,Daunert教授和她的学生将使用孢子形成细菌开发用于各种目标分析物的基因工程全细胞传感系统。 然后,它们将产生孢子作为保存、储存和运输传感细胞的手段。 随后,他们将把这些休眠的传感器整合到一个小型化的光盘状微流体平台中。 此外,他们将把它们放在纸条上,作为一个简单的即用型配置。 这两种系统都可以运输到田间,在田间孢子可以萌发成营养细胞并用于传感。 Daunert教授设想,这种新策略可以扩大全细胞生物传感器在现场分析中的使用,不仅在温和的环境中,而且在以前无法使用的环境和应用中。 其中包括沙漠、极地和太空等极端环境,以及用于生物恐怖主义的化学战剂的遥感。 这项工作对发展中国家的影响可能很大,因为这些国家需要快速、简单和廉价的分析工具来监测人类健康和环境,而不利的气候条件和不适当的分配和储存设施往往造成限制。 该项目将为学生提供良好的培训机会,在具有高度社会影响力的前沿多学科研究领域。
项目成果
期刊论文数量(0)
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Sylvia Daunert其他文献
Shining light on biosensors: Chemiluminescence and bioluminescence in enabling technologies
聚焦生物传感器:使能技术中的化学发光与生物发光
- DOI:
10.1016/j.trac.2024.117975 - 发表时间:
2024-11-01 - 期刊:
- 影响因子:12.000
- 作者:
Barbara Roda;Sapna K. Deo;Gregory O'Connor;Michael Moraskie;Stefano Giordani;Valentina Marassi;Aldo Roda;Sylvia Daunert - 通讯作者:
Sylvia Daunert
Probing a myth: does the younger generation of scientists have it easier?
- DOI:
10.1007/s00216-012-6083-7 - 发表时间:
2012-05-30 - 期刊:
- 影响因子:3.800
- 作者:
Sapna Deo;Yinan Wei;Sylvia Daunert - 通讯作者:
Sylvia Daunert
Exhaled breath analysis applications for evaluating occupational and environmental exposures
呼出气分析在评估职业和环境暴露方面的应用
- DOI:
10.1016/j.trac.2024.117787 - 发表时间:
2024-08-01 - 期刊:
- 影响因子:12.000
- 作者:
Umer Bakali;Chitvan Killawala;Evelise Monteagudo;Emre Dikici;Sapna K. Deo;Sylvia Daunert - 通讯作者:
Sylvia Daunert
Exosomal HPV oncogene and PD-L1 in cervical cancer: potential biomarkers for monitoring disease progression and treatment response
- DOI:
10.1016/s0090-8258(21)00938-0 - 发表时间:
2021-08-01 - 期刊:
- 影响因子:
- 作者:
Molly Roy;Angel Tabuyo-Martin;Yu-Ping Yang;Brian Slomovitz;Matthew Schlumbrecht;Sylvia Daunert - 通讯作者:
Sylvia Daunert
CHARACTERIZING REPRODUCTIVE TRACT MICROBIOME THROUGH SHOTGUN METAGENOMIC SEQUENCING AT TIME OF IVF, A PILOT STUDY
- DOI:
10.1016/j.fertnstert.2021.07.314 - 发表时间:
2021-09-01 - 期刊:
- 影响因子:
- 作者:
Jonah D. Bardos;Greg O'Connor;Fethiye Sinem Karipcin;Alan H. DeCherney;Micah J. Hill;Sylvia Daunert;Armando Hernendez-Reys;Michael Paidas - 通讯作者:
Michael Paidas
Sylvia Daunert的其他文献
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{{ truncateString('Sylvia Daunert', 18)}}的其他基金
EAGER SitS:Smart Long-Lived Biosensors for Soil Monitoring Using Engineered Spores
EAGER SitS:使用工程孢子进行土壤监测的智能长寿命生物传感器
- 批准号:
1841419 - 财政年份:2019
- 资助金额:
$ 40.5万 - 项目类别:
Standard Grant
Protein displayed spores and spore ghosts platforms for detection and biotransformation
用于检测和生物转化的蛋白质展示孢子和孢子幽灵平台
- 批准号:
1506740 - 财政年份:2015
- 资助金额:
$ 40.5万 - 项目类别:
Standard Grant
Collaborative Research: Extended Life Implantable Biosensor Platform
合作研究:延长寿命的植入式生物传感器平台
- 批准号:
1142376 - 财政年份:2011
- 资助金额:
$ 40.5万 - 项目类别:
Continuing Grant
Spore-Based Biosensing Systems: A Stabilized Dormant-Active Approach to Whole-Cell Biosensors
基于孢子的生物传感系统:全细胞生物传感器的稳定休眠活性方法
- 批准号:
1138278 - 财政年份:2010
- 资助金额:
$ 40.5万 - 项目类别:
Standard Grant
Collaborative Research: Extended Life Implantable Biosensor Platform
合作研究:延长寿命的植入式生物传感器平台
- 批准号:
0801778 - 财政年份:2008
- 资助金额:
$ 40.5万 - 项目类别:
Continuing Grant
Whole Cell and Regulatory Protein-Based Sensors in Bioanalysis
生物分析中基于全细胞和调节蛋白的传感器
- 批准号:
0416553 - 财政年份:2004
- 资助金额:
$ 40.5万 - 项目类别:
Standard Grant
Optical Sensing Based on Inducible Bacterial Luminescence
基于诱导细菌发光的光学传感
- 批准号:
9820808 - 财政年份:1999
- 资助金额:
$ 40.5万 - 项目类别:
Continuing Grant
Optical Sensors Based on Inducible Bacterial Bioluminescence
基于诱导细菌生物发光的光学传感器
- 批准号:
9502299 - 财政年份:1995
- 资助金额:
$ 40.5万 - 项目类别:
Continuing Grant
Research Experiences for Undergraduates at University of Kentucky
肯塔基大学本科生的研究经历
- 批准号:
9300384 - 财政年份:1993
- 资助金额:
$ 40.5万 - 项目类别:
Continuing Grant
Research Experiences for Undergraduates in Chemistry at the University of Kentucky
肯塔基大学化学专业本科生的研究经历
- 批准号:
9000653 - 财政年份:1990
- 资助金额:
$ 40.5万 - 项目类别:
Continuing Grant
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