Optical Sensing Based on Inducible Bacterial Luminescence

肯塔基大学的Sylvia Daunert教授的这个项目得到了分析和表面化学计划的支持，从开发基因工程细菌开始，然后将其用于光学传感系统，应用于环境分析中的问题。这些细菌含有报告基因，当接触到目标分析物时，这些基因会提供特定波长的光发射。开发了一种内部背景校正系统，允许扩展光谱方法以实现低水平的检测。探索了基于细菌的传感器的布置，用于在流动的气流和静态溶液中检测多分析物。细菌基因被修饰，使它们在接触到痕量目标化合物时发出光(发光)。例如，通过插入包含设计的细菌系统的传感器来分析水样。易于测量的光输出提供了分析物浓度的快速指示。肯塔基大学的Sylvia Daunert教授将研究如何修改细菌，使其包含发光基因，以及如何将它们整合到传感器中。

This project of Professor Sylvia Daunert at the University of Kentucky, supported by the Analytical and Surface Chemistry Program, begins with development of genetically engineered bacteria that are then used in optical sensing systems applied to problems in environmental analysis. The bacteria contain reporter genes that provide light emission of specific wavelength when exposed to the targeted analyte. An internal background correction system is developed that allows extension of the spectroscopic method to achieve low levels of detection. Arrangements of bacteria-based sensors are explored for multianalyte sensing in flow streams and in static solutions.Bacterial genes are modified such that they give off light (luminesce) when exposed to trace levels of targeted compounds. For example, a water sample is analyzed by insertion of a sensor that contains the designed bacterial system. The easily measured light output provides a rapid indication of the concentration of analyte. Professor Sylvia Daunert at the University of Kentucky will study how to modify the bacteria so that they contain the luminescent genes, and how to incorporate them into sensors.