Optical Sensing Based on Inducible Bacterial Luminescence

基于诱导细菌发光的光学传感

• 批准号: 9820808
• 负责人: Sylvia Daunert
• 金额: $ 37.4万
• 依托单位: University of Kentucky Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2005-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9820808&HistoricalAwards=false
• 关键词: Optical; Sensing; Based; Inducible; Bacterial

This project of Professor Sylvia Daunert at the University of Kentucky, supported by the Analytical and Surface Chemistry Program, begins with development of genetically engineered bacteria that are then used in optical sensing systems applied to problems in environmental analysis. The bacteria contain reporter genes that provide light emission of specific wavelength when exposed to the targeted analyte. An internal background correction system is developed that allows extension of the spectroscopic method to achieve low levels of detection. Arrangements of bacteria-based sensors are explored for multianalyte sensing in flow streams and in static solutions.Bacterial genes are modified such that they give off light (luminesce) when exposed to trace levels of targeted compounds. For example, a water sample is analyzed by insertion of a sensor that contains the designed bacterial system. The easily measured light output provides a rapid indication of the concentration of analyte. Professor Sylvia Daunert at the University of Kentucky will study how to modify the bacteria so that they contain the luminescent genes, and how to incorporate them into sensors.

肯塔基州大学的Sylvia Daunert教授的这个项目得到了分析和表面化学项目的支持，从开发基因工程细菌开始，然后将其用于应用于环境分析问题的光学传感系统。 细菌含有当暴露于目标分析物时提供特定波长的光发射的报告基因。 开发了一种内部背景校正系统，该系统允许扩展光谱方法以实现低水平的检测。 基于细菌的传感器的排列被探索用于流动流和静态溶液中的多分析物传感。细菌基因被修饰，使得它们在暴露于痕量水平的目标化合物时发光（发光）。 例如，通过插入包含所设计的细菌系统的传感器来分析水样。 容易测量的光输出提供了分析物浓度的快速指示。 肯塔基州大学的Sylvia Daunert教授将研究如何修改细菌，使它们包含发光基因，以及如何将它们纳入传感器。