Optical Sensors Based on Inducible Bacterial Bioluminescence

基于诱导细菌生物发光的光学传感器

• 批准号: 9502299
• 负责人: Sylvia Daunert
• 金额: $ 22.35万
• 依托单位: University of Kentucky Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-04-01 至 1998-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9502299&HistoricalAwards=false
• 关键词: Optical; Sensors; Based; Inducible; Bacterial

This Faculty Early Career Development (CAREER) project, supported in the Analytical and Surface Chemistry Program, focusses on the development of highly selective and sensitive fiber optic biosensors that employ genetically designed bacteria as the sensing element. During the tenure of this three-year continuing grant, Professor Daunert and her students at the University of Kentucky will develop recombinant bacteria that luminesce in the presence of toxic heavy metals such as chromium, arsenic, and nickel, incorporate these bacteria as the active elements in fiber optic sensors, and optimize the resulting devices. Specific targets include (a) coupling the mechanism of resistance of bacteria to chromate, arsenic, or nickel with the luciferase bioluminescence system; (b) preparing bacterial systems that will bioluminesce in the presence of the targeted metal; and (c) developing biosensors using these engineered bacteria and validating their performance with real samples. Also during this grant period, Professor Daunert will pursue an educational development plan that includes broad involvement of undergraduates in research, increased public appreciation of chemistry, and the attraction, mentoring, and retention of undergraduate students from underrepresented demographic groups. The development of more rapid and accurate techniques for monitoring low-level concentrations of toxic chemicals is important to progress in environmental protection. By combining the tools of modern molecular biology with fiber optic sensor technology, this CAREER research project should lead to new highly selective and sensitive probes for low concentrations of toxic heavy metals. The educational aspects of this CAREER project will expand the role of research in the training of undergraduates at the University of Kentucky and should increase the participation of women and underrepresented minorities in science at that institution.

该学院早期职业发展（CAREER）项目，在分析和表面化学计划的支持下，专注于开发高度选择性和敏感的光纤生物传感器，该传感器采用遗传设计的细菌作为传感元件。 在为期三年的持续资助期间，肯塔基州大学的Daunert教授和她的学生将开发重组细菌，这些细菌在有毒重金属（如铬，砷和镍）的存在下发光，将这些细菌作为光纤传感器中的活性元素，并优化所得设备。 具体目标包括（a）将细菌对铬酸盐、砷或镍的抗性机制与荧光素酶生物发光系统偶联;（B）制备在目标金属存在下生物发光的细菌系统;以及（c）使用这些工程细菌开发生物传感器并用真实的样品验证它们的性能。 此外，在此赠款期间，Daunert教授将推行教育发展计划，包括本科生广泛参与研究，提高公众对化学的认识，以及吸引，指导和保留来自代表性不足的人口群体的本科生。 发展更迅速和更准确的技术来监测低浓度的有毒化学品对环境保护的进展很重要。 通过将现代分子生物学工具与光纤传感器技术相结合，该CAREER研究项目将为低浓度的有毒重金属提供新的高选择性和灵敏度的探针。 这个职业项目的教育方面将扩大研究在肯塔基州大学本科生培训中的作用，并应增加妇女和代表性不足的少数民族在该机构的科学参与。