Protein displayed spores and spore ghosts platforms for detection and biotransformation

用于检测和生物转化的蛋白质展示孢子和孢子幽灵平台

• 批准号: 1506740
• 负责人: Sylvia Daunert
• 金额: $ 36万
• 依托单位: University of Miami School of Medicine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-06-15 至 2019-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1506740&HistoricalAwards=false
• 关键词: Protein; displayed; spores; spore; ghosts

In this project, funded by the Chemical Measurement and Imaging Program of the Chemistry Division, the multidisciplinary team of Dr. Sylvia Daunert and Dr. Sapna Deo at the Miller School of Medicine of the University of Miami will design and prepare sensors and bioremediation systems that take advantage of the natural hardiness and ruggedness of bacterial spores to develop platforms in which biosensing-biotransformation protein reagents are displayed on the spore surface. These surface-displayed proteins are employed for sensing, biotransformation, and immobilization purposes and employed to develop paper-based detection platforms. This strategy provides with portable, easy-to-use, rapid, cost-effective, and stable biosensing and biotransformation systems with a wide range of potential applications in the environmental field. The broader impacts are addressed not only through the scientific impact of the work but also through educational and outreach activities. Students are trained to be effective communicators by classical methods and by learning to make short films and videos featuring their own research. Academic and industrial collaborations expose the students to global science. Further, a new generation of physician scientists are trained in critical analytical thinking, problem solving and in research through a 'Molecular Medicine' Pathway whereby medical students perform laboratory work and participate in research group activities. The goal is that medical graduates gain the necessary skills to become successful researchers in academic medicine or clinicians who can tackle medical problems from their root at the molecular level. This project advances the field of bioanalytical chemistry by creating powerful biosensing tools that are stable in various extreme environmental conditions for prolonged periods of time and can be integrated into easy-to-use analytical devices with a rapid response. Natural ruggedness and successful genetic manipulation allows for spores to provide more stable biosensing systems with a wide range of applications in environmental and biomedical sciences by facilitating the transport and expanding on-site use, service lives, and storage of biosensors. The project is interdisciplinary in nature with elements of bioanalytical chemistry, molecular biology, sensor development and miniaturization, and biomedical/environmental science.

在这个由化学部化学测量和成像计划资助的项目中，迈阿密大学米勒医学院的Sylvia Daunert博士和Sapna Deo博士组成的多学科团队将设计和制备传感器和生物修复系统，利用细菌孢子的天然坚韧性和坚固性开发生物传感平台-生物转化蛋白试剂展示在孢子表面。这些表面展示的蛋白质用于传感、生物转化和固定化目的，并用于开发基于纸的检测平台。这种策略提供了便携式，易于使用，快速，成本效益高，稳定的生物传感和生物转化系统，在环境领域具有广泛的潜在应用。不仅通过工作的科学影响，而且通过教育和外联活动，应对更广泛的影响。学生通过经典的方法和学习制作短片和视频来训练成为有效的沟通者。学术和工业合作使学生接触全球科学。此外，新一代的医生科学家通过“分子医学”途径接受批判性分析思维，解决问题和研究方面的培训，医学生通过该途径进行实验室工作并参加研究小组活动。目标是使医学毕业生获得必要的技能，成为成功的学术医学研究人员或临床医生，他们可以从分子水平上解决医学问题。该项目通过创建强大的生物传感工具来推进生物分析化学领域，这些工具在各种极端环境条件下长时间保持稳定，并且可以集成到易于使用的快速响应分析设备中。天然的坚固性和成功的遗传操作允许孢子提供更稳定的生物传感系统，通过促进运输和扩大生物传感器的现场使用、使用寿命和储存，在环境和生物医学科学中具有广泛的应用。该项目具有跨学科性质，涉及生物分析化学、分子生物学、传感器开发和微型化以及生物医学/环境科学。