Protein displayed spores and spore ghosts platforms for detection and biotransformation

在这个由化学系化学测量和成像计划资助的项目中，迈阿密大学米勒医学院的Sylvia Daunert博士和Sapna Deo博士组成的多学科团队将设计和制备传感器和生物修复系统，利用细菌孢子的天然坚韧和粗犷来开发在孢子表面展示生物传感-生物转化蛋白质试剂的平台。这些表面展示的蛋白质用于传感、生物转化和固定化目的，并用于开发纸基检测平台。这一战略提供了便携、易用、快速、经济有效和稳定的生物传感和生物转化系统，在环境领域具有广泛的潜在应用。更广泛的影响不仅通过工作的科学影响来解决，而且还通过教育和外联活动来解决。通过经典的方法和学习制作以他们自己的研究为特色的短片和视频，学生们被训练成有效的沟通者。学术和产业合作使学生接触到全球科学。此外，新一代内科科学家在批判性分析思维、解决问题和研究方面的培训通过“分子医学”途径进行，医学生通过这种途径执行实验室工作并参与研究小组活动。目标是医学毕业生获得必要的技能，成为成功的学术医学研究人员或临床医生，能够从分子水平上从根本上解决医学问题。该项目通过创建强大的生物传感工具来推动生物分析化学领域的发展，这些工具在各种极端环境条件下可以长时间稳定，并可以集成到易于使用的分析设备中，反应迅速。天然的坚固性和成功的基因操作使孢子能够提供更稳定的生物传感系统，通过促进生物传感器的运输和扩大现场使用、使用寿命和储存，在环境和生物医学科学中有广泛的应用。该项目是跨学科的，涉及生物分析化学、分子生物学、传感器开发和微型化以及生物医学/环境科学。

In this project, funded by the Chemical Measurement and Imaging Program of the Chemistry Division, the multidisciplinary team of Dr. Sylvia Daunert and Dr. Sapna Deo at the Miller School of Medicine of the University of Miami will design and prepare sensors and bioremediation systems that take advantage of the natural hardiness and ruggedness of bacterial spores to develop platforms in which biosensing-biotransformation protein reagents are displayed on the spore surface. These surface-displayed proteins are employed for sensing, biotransformation, and immobilization purposes and employed to develop paper-based detection platforms. This strategy provides with portable, easy-to-use, rapid, cost-effective, and stable biosensing and biotransformation systems with a wide range of potential applications in the environmental field. The broader impacts are addressed not only through the scientific impact of the work but also through educational and outreach activities. Students are trained to be effective communicators by classical methods and by learning to make short films and videos featuring their own research. Academic and industrial collaborations expose the students to global science. Further, a new generation of physician scientists are trained in critical analytical thinking, problem solving and in research through a 'Molecular Medicine' Pathway whereby medical students perform laboratory work and participate in research group activities. The goal is that medical graduates gain the necessary skills to become successful researchers in academic medicine or clinicians who can tackle medical problems from their root at the molecular level. This project advances the field of bioanalytical chemistry by creating powerful biosensing tools that are stable in various extreme environmental conditions for prolonged periods of time and can be integrated into easy-to-use analytical devices with a rapid response. Natural ruggedness and successful genetic manipulation allows for spores to provide more stable biosensing systems with a wide range of applications in environmental and biomedical sciences by facilitating the transport and expanding on-site use, service lives, and storage of biosensors. The project is interdisciplinary in nature with elements of bioanalytical chemistry, molecular biology, sensor development and miniaturization, and biomedical/environmental science.