Collaborative Research: Extended Life Implantable Biosensor Platform

目的：开发一种基于葡萄糖结合蛋白(GBP)的微型生物传感平台，能够连续、可靠地测量低血糖、正常血糖和高血糖范围内的血糖水平。该平台将由三个部分组成：基于模型酶葡萄糖氧化酶(GOX)对葡萄糖的电化学检测的葡萄糖传感阵列、独立电源和遥测系统。这种生物传感阵列的性能将与成熟的Gox葡萄糖生物传感器进行比较。智力价值：该项目具有超越血糖监测的意义，因为相同的微型生物传感器平台可以与广泛的其他生物传感器一起用于其他应用。此外，对一系列分析物具有选择性的多个传感器可以集成到单个设备的不同储存库中，从而允许测量多个分析物。此外，该平台还可用作响应性药物输送系统。这样的系统将装载了微型传感器的水库与含药物的水库结合在一起。最后，拟议的平台应该导致能够在个性化医疗中找到应用的技术。更广泛的影响：这项工作将涉及研究生和本科生。这样的工作将促进跨学科的互动，并将为学生提供设计和开发新生物技术所需的技能和知识。研究人员计划广泛传播这项工作的结果，并提高公众对糖尿病研究、生物传感、混合系统和设备微型化的认识。这将通过参加讲习班和外联方案来实现。新开发的技术将在生物工程课程中重点介绍。

Objective:The objective is to develop a microfabricated biosensing platform based on Glucose Binding Protein (GBP) that continuously and reliably measures glucose levels in the hypoglycemic, normal, and hyperglycemic range. The platform will consist of three components: a glucose-sensing array based on the electrochemical detection of glucose using the model enzyme glucose oxidase (GOx), an independent power supply, and a telemetry system. The performance of this biosensing array will be compared with that of the well-established GOx glucose biosensor. Intellectual Merit:The project has significance beyond blood glucose monitoring, since identical miniaturized biosensing platforms can be utilized for other applications with a wide range of other biosensors. In addition, multiple sensors selective to a series of analytes can be incorporated in different reservoirs within a single device, allowing the measurement of multiple analytes. Moreover, this platform can be utilized as a responsive drug delivery system. Such a system integrates reservoirs loaded with miniature sensors with drug-containing reservoirs. Finally, the proposed platforms should result in enabling technologies that will find applications in individualized medicine. Broader Impact:The work will involve students at both, the graduate and undergraduate levels. Such work will foster interdisciplinary interactions and will provide the students with the necessary skills and knowledge for the design and development of novel biotechnologies. The investigators plan to broadly disseminate the results of this work and raise the awareness of the general public in diabetes research, biosensing, hybrid systems, and device miniaturization. This will be accomplished through participation in workshops and outreach programs. The newly developed technologies will be highlighted in bioengineering courses.