Acquisition of an HPLC-MS Instrument for Research and Training

小石城阿肯色大学化学系将获得重大研究仪器(MRI)计划颁发的这一奖项，获得一台带有光电二极管阵列紫外-可见光检测器的高性能液相色谱仪，随后将获得一台带有离子陷阱质谱仪的电喷雾电离/大气压化学电离(HPLC-MS)。该设备将加强如下领域的研究：a)调查植物多酚的化学生物相互作用；b)研究光合作用生物适应高光条件对细胞造成氧化损害的分子机制；c)分析食肉动物粪便的胆汁酸谱以鉴定美洲狮；d)金黄色葡萄球菌谷胱甘肽过氧化物酶同系物的生化活性；e)土壤微生物对农药和除草剂的生物降解；f)用质谱仪对蛋白质和多肽进行测序；以及g)新型水溶性富勒烯具有增强的抗氧化性能。高效液相色谱-质谱法是一种用于复杂混合物的分离和分析的极其强大的技术。这一仪器将大大加强阿肯色大学小石城分校和菲兰德史密斯学院的科学研究，这是一所主要是本科生的机构，有大量的少数族裔入学。这些研究将在许多领域产生坚实的影响，包括环境科学和生物化学。

With this award from the Major Research Instrumentation (MRI) Program, the Department of Chemistry at the University of Arkansas in Little Rock will acquire a high performance liquid chromatograph with a photodiode-array UV-visible detector, followed by an electrospray ionization/atmospheric pressure chemical ionization with ion-trap mass spectrometer (HPLC-MS). This equipment will enhance research in a number of areas including a) investigation of the chemico-biological interactions of plant polyphenols; b) investigation of the molecular mechanisms by which photosynthetic organisms acclimate to high light conditions that cause oxidative damage to cells; c) analysis of bile acid profiles of carnivore scats for the identification of mountain lion; d) biochemical activity of glutathione peroxidase homologs from Staphylococcus aureus; e) biodegradation of pesticides and herbicides by soil-borne micro-organisms; f) sequencing of proteins and peptides by mass spectrometry; and g) novel water-soluble fullerenes with enhanced anti-oxidative properties . High performance liquid chromatography with mass spectrometric detection is an extremely powerful technique used for the separation and analysis of complex mixtures. This instrument will substantially strengthen scientific research at the University of Arkansas at Little Rock and Philander Smith College, a primarily undergraduate institution with significant minority enrollment. These studies will have a solid impact in a number of areas, including environmental sciences and biochemistry.