Interactions of the TCR co-receptors and p56LCK in an Ectothermic Model

变温模型中 TCR 共受体和 p56LCK 的相互作用

• 批准号: 0453924
• 负责人: John Hansen
• 金额: $ 3.4万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0453924&HistoricalAwards=false
• 关键词: Interactions; TCR; co; receptors; p56LCK

The vertebrate immune system serves to protect individuals from disease and neoplasm. It is composed of innate and adaptive characteristics that have been well-studied in mammals but very little is known about the processes governing the development of the immune repertoire in lower vertebrates, such as fish. Thus a fish model can provide an evolutionary perspective into the processes that have shaped the vertebrate immune system. Cytotoxic and helper T-cells are derived from a common T-cell precursor that commits to either of these two lineages after receiving specific signals in the thymus through a process known as T-cell development. Two different models have been put forth to explain T-cell development in mammals. It is the goal of this study to examine this process in rainbow trout to provide a comparative approach for examining the two models. This study will assess the pathways and signals that lead to development of the two T-cell subsets in fish. Specifically, this proposal will allow for the development of tools such as monoclonal antibodies that can be used to follow the developmental program that trout T-cells utilize during their development.This project will also serve to train and educate high school and undergraduate students in critical thought and current techniques in molecular biology and immunology. It will also show students the value of using comparative studies to solve fundamental paradigms in biology. Overall, the major objective of this project is to provide a basic understanding of the processes governing T-cell development in fish with a long-term goal of ascertaining the extent of conservation for this process in all vertebrates. These studies will have ramifications for research dealing the development of T-cells in mammals while also providing basic research and tools that can be applied to the preservation and maintenance of salmonid fish.

脊椎动物的免疫系统用于保护个体免受疾病和肿瘤的侵害。 它由先天和适应性特征组成，这些特征在哺乳动物中已得到充分研究，但对低等脊椎动物（如鱼类）免疫库发育的过程知之甚少。 因此，鱼类模型可以为脊椎动物免疫系统的形成过程提供一个进化的视角。细胞毒性T细胞和辅助性T细胞来源于一种共同的T细胞前体，该T细胞前体在胸腺中通过称为T细胞发育的过程接收特定信号后致力于这两种谱系中的任一种。 有两种不同的模型被提出来解释哺乳动物的T细胞发育。 这是本研究的目标，以检查这一过程中虹鳟鱼提供一个比较的方法来检查这两种模式。 这项研究将评估导致鱼类两种T细胞亚群发育的途径和信号。 具体来说，该提案将允许开发工具，如单克隆抗体，可用于跟踪鳟鱼T细胞在其发育过程中使用的发育程序。该项目还将用于培养和教育高中和本科生的批判性思维和分子生物学和免疫学的最新技术。它还将向学生展示使用比较研究解决生物学基本范式的价值。 总体而言，该项目的主要目标是提供对鱼类T细胞发育过程的基本了解，长期目标是确定所有脊椎动物中这一过程的保护程度。 这些研究将对哺乳动物T细胞发育的研究产生影响，同时也提供了可用于鲑鱼保存和维护的基础研究和工具。