Development of a high sensitivity optical microscopy based on Nüvü's electron-multiplying CCD camera for real-time imaging of sub-threshold membrane potential dynamics

开发基于 Nüvü 电子倍增 CCD 相机的高灵敏度光学显微镜，用于亚阈值膜电位动态的实时成像

• 批准号: 530850-2018
• 负责人: Liang, Jinyang
• 金额: $ 1.82万
• 依托单位: Institut national de la recherche scientifique
• 依托单位国家: 加拿大
• 项目类别: Engage Grants Program
• 财政年份: 2018
• 资助国家: 加拿大
• 起止时间: 2018-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=657571
• 关键词: Development; sensitivity; optical; microscopy; based

This Engage project aims to form an industry-university partnership between INRS and Nüvü Caméra on high**sensitivity optical neuroimaging. The overall objective of this six-month project is to demonstrate optical**voltage imaging using an electron-multiplying (EM) CCD camera from Nüvü under a microscopic setting. Four**milestones are proposed, which involves assembling the hardware, writing the software, and conducting**proof-of-concept experiments. Prof. Liang's group and Nüvü have necessary and complementary expertise that**encompasses all research tasks proposed in this application. Both sides are committed to frequent interaction**and communication to ensure the progress of this project and to facilitate the technology transfer.**Aiming to demonstrate the feasibility of Nüvü's EMCCD camera on a major-brand microscope platform, this**project is expected to significantly benefit Nüvü by boosting up its sales to solidify its current technical team**with the potential to open new positions, and by enhancing its reputation in the biomedical field through the**visibility and publicity of the disseminated results. In addition, this project has compelling scientific merits,**because Nüvü's EMCCD camera can potentially provide a considerably better sensitivity in measurement. All**these activities will support the future knowledge-based economy of Canada.

该Engage项目旨在形成INRS和Nüvü Caméra之间在高 ** 灵敏度光学神经成像方面的产学研合作伙伴关系。这个为期六个月的项目的总体目标是在显微镜下使用Nüvü的电子倍增（EM）CCD相机演示光学 ** 电压成像。提出了四个里程碑，包括组装硬件，编写软件和进行概念验证实验。Liang教授的团队和Nüvü拥有必要和互补的专业知识，** 涵盖了本申请中提出的所有研究任务。双方承诺经常进行互动 ** 和沟通，以确保该项目的进展，并促进技术转让 **。该 ** 项目旨在展示Nüvü的EMCCD相机在主要品牌显微镜平台上的可行性，预计将通过提高销售额来巩固其现有的技术团队 ** 并有可能开设新职位，以及通过传播结果的可见性和宣传来提高其在生物医学领域的声誉，从而使Nüvü受益匪浅。此外，该项目具有令人信服的科学价值，** 因为Nüvü的EMCCD相机可以提供更高的测量灵敏度。所有这些活动都将支持加拿大未来的知识经济。