Novel Multi-Photon Fluorescence and Raman Near-Field Optical Microscope: A Versatile Sub-20nm Optical Resolution Spectroscopic Tool for Biological Applications

新型多光子荧光和拉曼近场光学显微镜：用于生物应用的多功能亚 20 纳米光学分辨率光谱工具

• 批准号: 0500812
• 负责人: Erik Sanchez
• 金额: $ 52.74万
• 依托单位: Portland State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-15 至 2009-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0500812&HistoricalAwards=false
• 关键词: Novel; Multi; Photon; Fluorescence; Raman

This award is for the development of an instrument with an anticipated optical spatial resolution below 20 nm and with simultaneous topographical information. The instrument will also have multiple methods of detection of both electronic state and vibrational bond information for single-molecule identification. The microscope's ability to image in solution with these capabilities will be unique. The microscope will image the fluorescence from almost any visible chromophore without changing filters or excitation wavelength, an aspect that will dramatically simplify the optical system. This proposed instrument will improve upon the nominal 75 nm resolution limit of today's NSOMs to a level that will allow identification of individual closely packed proteins in a biologically friendly environment. The versatility of the instrument will permit analyzing more than the biological samples described within this proposal. Prototypes built by the PI and collaborators have imaged carbon single walled nanotubes (SWNT) as well as novel polymer systems at sub 25 nm spatial optical resolutions. The proposed instrument can easily be adapted for use in materials science for analyzing structures of semiconductor wires and other inorganic 1-D and 2-D materials on the nanoscale, as well as signal detection improvement schemes for nanometrology.In the process of development, the proposed instrument will have the added benefit of bringing together researchers in mathematics (modeling), biology, physics, chemistry, and electrical engineering. The broad understanding necessary to develop cutting edge NSOM instrumentation will benefit students as well by teaching them very strong technical skills, cross-disciplinary training, and the ability to bring different fields together to answer important scientific questions. After completion this microscope will be available to answer other biological and materials research questions, and there will be commercial possibilities.

该奖项旨在开发一种预期光学空间分辨率低于20 nm并同时提供地形信息的仪器。该仪器还将有多种方法检测电子状态和振动键信息，用于单分子鉴定。 该显微镜在具有这些功能的溶液中成像的能力将是独一无二的。该显微镜将成像荧光从几乎任何可见的发色团，而无需改变过滤器或激发波长，一个方面，将大大简化光学系统。 该拟议的仪器将改进后的标称75 nm分辨率限制今天的NSOM的水平，将允许识别个人紧密包装的蛋白质在一个生物友好的环境。该仪器的多功能性将允许分析本提案中描述的生物样品以外的更多样品。PI和合作者建造的原型已经成像了碳单壁纳米管（SWNT）以及低于25 nm空间光学分辨率的新型聚合物系统。该仪器可以很容易地应用于材料科学领域，用于分析半导体导线和其他无机纳米材料的结构，以及纳米计量学的信号检测改进方案。在开发过程中，该仪器还可以将数学（建模）、生物学、物理学、化学和电气工程领域的研究人员聚集在一起。开发尖端NSOM仪器所需的广泛理解也将使学生受益，教授他们非常强大的技术技能，跨学科培训以及将不同领域结合在一起回答重要科学问题的能力。完成后，这种显微镜将可用于回答其他生物和材料研究问题，并将有商业可能性。