Molecular analysis of MOS7/Nup88-regulated immune response pathways in Arabidopsis.

拟南芥中 MOS7/Nup88 调节的免疫反应途径的分子分析。

• 批准号: 170233861
• 负责人: Professor Dr. Marcel Wiermer
• 金额: --
• 依托单位: Arbeitsgruppe Wiermer - Biochemie der Pflanzen-Mikroben Interaktionen
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/170233861?language=en
• 关键词: Molecular; analysis; MOS7; Nup88; regulated

Plant innate immunity against microbial infection is a dynamic process that requires stimulus-dependent spatial and temporal action of its defence signalling components. Translocation of intracellular immune receptors and regulators across the nuclear envelope are recently uncovered aspects of plant defence signal relay. Macromolecular trafficking between the cytoplasm and nucleus exclusively occurs through nuclear pore complexes and typically depends on transport receptors. We recently identified a number of nuclear pore and nucleocytoplasmic trafficking components that are essential for distinct immune responses. However, the molecular mechanisms that facilitate spatial communication between the cytoplasm and the nucleoplasm are just beginning to emerge. My proposed research efforts are directed towards understanding the molecular function of host components and signalling mechanisms specifically involved in defencerelated nucleocytoplasmic transport. The studies include in planta affinity purification approaches, yeast two-hybrid analyses, forward and reverse genetics to identify novel biochemical and genetic interactors that will be characterised in detail. Together, these studies will reveal important information on the intricate regulation of cellular plant immune responses. This should provide insights as to how the plant immune system can be modified to improve plant protection.

植物抗微生物感染的天然免疫是一个动态过程，需要其防御信号组分的刺激依赖性空间和时间作用。细胞内免疫受体和调节因子的跨核膜转运是最近发现的植物防御信号传递的方面。细胞质和细胞核之间的大分子运输仅通过核孔复合物发生，并且通常依赖于转运受体。我们最近确定了一些核孔和核质运输组件是必不可少的不同的免疫反应。然而，促进细胞质和核质之间空间通讯的分子机制才刚刚开始出现。我建议的研究工作是针对理解宿主组件的分子功能和信号机制，特别是参与防御相关的核质运输。这些研究包括植物亲和纯化方法，酵母双杂交分析，正向和反向遗传学，以确定新的生化和遗传相互作用，将详细描述。总之，这些研究将揭示细胞植物免疫反应的复杂调节的重要信息。这将为如何修改植物免疫系统以改善植物保护提供见解。

项目成果

Nucleoporin NUP88/MOS7 is required for manifestation of phenotypes associated with the Arabidopsis CHITIN ELICITOR RECEPTOR KINASE1 mutant cerk1–4

拟南芥几丁质激发受体激酶 1 突变体 cerk1â4 相关表型的表现需要核孔蛋白 NUP88/MOS7

• DOI: 10.1080/15592324.2017.1313378
• 发表时间: 2017
• 期刊: Plant Signaling & Behavior
• 影响因子: 2.9
• 作者: Genenncher;Petutschnig;Wiermer
• 通讯作者: Wiermer

The putative kinase substrate MUSE7 negatively impacts the accumulation of NLR proteins

• DOI: 10.1111/tpj.13454
• 发表时间: 2017-03-01
• 期刊: PLANT JOURNAL
• 影响因子: 7.2
• 作者: Johnson, Kaeli C. M.;Zhao, Jin;Li, Xin
• 通讯作者: Li, Xin

E3 ligase SAUL1 serves as a positive regulator of PAMP-triggered immunity and its homeostasis is monitored by immune receptor SOC3.

• DOI: 10.1111/nph.14678
• 发表时间: 2017-07
• 期刊: The New phytologist
• 作者: Meixuezi Tong;Tanja Kotur;Wanwan Liang;Katja Vogelmann;T. Kleine;D. Leister;Catharina Brieske;Shuhua Yang;Daniel Lüdke;M. Wiermer;Yuelin Zhang;Xin Li;S. Hoth