Molecular analysis of MOS7/Nup88-regulated immune response pathways in Arabidopsis.

植物对微生物侵染的天然免疫是一个动态的过程，需要其防御信号成分在刺激下的空间和时间作用。细胞内免疫受体和调节器跨核膜的移位是最近发现的植物防御信号传递的方面。细胞质和细胞核之间的大分子运输仅通过核孔复合体发生，通常依赖于转运受体。我们最近发现了一些核孔和核质转运成分，它们对不同的免疫反应是必不可少的。然而，促进细胞质和核质之间空间通讯的分子机制才刚刚开始出现。我建议的研究工作是为了了解宿主成分的分子功能和信号机制，特别是涉及防御相关的核质运输。这些研究包括植物亲和纯化方法、酵母双杂交分析、正向和反向遗传学，以确定将详细描述的新的生化和遗传相互作用因素。总之，这些研究将揭示关于细胞植物免疫反应的复杂调控的重要信息。这应该为如何修改植物免疫系统以改善植物保护提供洞察力。

Plant innate immunity against microbial infection is a dynamic process that requires stimulus-dependent spatial and temporal action of its defence signalling components. Translocation of intracellular immune receptors and regulators across the nuclear envelope are recently uncovered aspects of plant defence signal relay. Macromolecular trafficking between the cytoplasm and nucleus exclusively occurs through nuclear pore complexes and typically depends on transport receptors. We recently identified a number of nuclear pore and nucleocytoplasmic trafficking components that are essential for distinct immune responses. However, the molecular mechanisms that facilitate spatial communication between the cytoplasm and the nucleoplasm are just beginning to emerge. My proposed research efforts are directed towards understanding the molecular function of host components and signalling mechanisms specifically involved in defencerelated nucleocytoplasmic transport. The studies include in planta affinity purification approaches, yeast two-hybrid analyses, forward and reverse genetics to identify novel biochemical and genetic interactors that will be characterised in detail. Together, these studies will reveal important information on the intricate regulation of cellular plant immune responses. This should provide insights as to how the plant immune system can be modified to improve plant protection.