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Molecular analysis of nucleocytoplasmic signaling pathways in plant cellular immunity

植物细胞免疫核质信号通路的分子分析

基本信息

批准号：
206673514
负责人：
Professor Dr. Marcel Wiermer
金额：
--
依托单位：
Arbeitsgruppe Wiermer - Biochemie der Pflanzen-Mikroben Interaktionen
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2011
资助国家：
德国
起止时间：
2010-12-31 至 2015-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/206673514?language=en
关键词：
Molecular analysis nucleocytoplasmic signaling pathways

项目摘要

Plant cellular immune responses against microbial infection depend on dynamic signaling events across the nuclear envelope (NE) through nuclear pore complexes (NPCs). However, the molecular mechanisms and components that facilitate spatial communication between the cytoplasm and the nucleoplasm in plant immunity are poorly understood. Our proposed research efforts are directed towards understanding the molecular function of the host nuclear import receptor MOS6 (Importin 3) and three members of the Nup107-160 nuclear pore sub-complex, Nup160, Seh1 and MOS3 (Nup96), that are essential for disease resistance in Arabidopsis and implicated in defense-related nuclear protein import and mRNA export, respectively. In particular, we aim to identify and molecularly characterize MOS6 cargo proteins and interacting partners involved in disease response signaling, using a biochemical in planta affinity purification approach and to address potential functional redundancy between Importin  family members in plant defense. The second research project will analyze nucleoporin mutants of the Nup107-160 complex for defects in the export of specific subsets of mRNAs required for defense and characterize the functional relationship of Nup107-160 complex members in regulating plant immunity. Together, these studies will reveal important information on the intricate regulation of cellular plant immune responses by nucleocytoplasmic transport. This should provide insights as to how the plant immune system can be modified to improve plant protection.

植物抗微生物感染的细胞免疫应答依赖于通过核孔复合物（NPC）穿过核膜（NE）的动态信号事件。然而，在植物免疫中促进细胞质和核质之间空间通讯的分子机制和组分知之甚少。我们提出的研究工作旨在了解宿主核输入受体MOS 6（Importin β 3）和Nup 107 -160核孔子复合物的三个成员Nup 160，Seh 1和MOS 3（Nup 96）的分子功能，它们对拟南芥的抗病性至关重要，并分别参与防御相关的核蛋白输入和mRNA输出。特别是，我们的目标是确定和分子特征的MOS 6货物蛋白和相互作用的合作伙伴参与疾病反应信号，使用生物化学在植物亲和纯化方法，并解决潜在的功能冗余之间的Importin family members in plant defense。第二个研究项目将分析Nup 107 -160复合体的核孔蛋白突变体，以确定防御所需的特定mRNA子集的出口缺陷，并表征Nup 107 -160复合体成员在调节植物免疫中的功能关系。总之，这些研究将揭示核质运输的细胞植物免疫反应的复杂调节的重要信息。这将为如何修改植物免疫系统以改善植物保护提供见解。