Bioinorganic Enzymology of a Non-Heme Iron Hydroxylase
非血红素铁羟化酶的生物无机酶学
基本信息
- 批准号:0502391
- 负责人:
- 金额:$ 44万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2005
- 资助国家:美国
- 起止时间:2005-09-01 至 2008-08-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Dr. Mahdi Abu-Omar, Chemistry Department, Purdue University, is supported by the Inorganic, Bioinorganic and Organometallic Chemistry Program of the Chemistry Divison to study the activity and substrate recognition in phenylalanine hydroxylase (PAH), a mononuclear non-heme iron hydroxylase.The reactions of nitric oxide with ferrous PAH will be used to spectroscopically model and probe the catalytically relevant iron(II) coordination environment of the early stages of O2 binding and activation in non-heme hydroxylases. The molecular structure of the quaternary complex of the enzyme with nitric oxide will be determined. Drop coating deposition Raman spectroscopy will be utilized to characterize molecular changes in the enzyme as well as in the substrates upon binding to the active site.The function of an important enzyme, phenylalanine hydroxylase, which synthesizes an essential amino acid, tyrosine that is further metabolized to important neurotransmitters will be studied. The work will focus on how oxygen is activated at its iron-containing active site. Postdoctoral, graduate, and undergraduate students will be educated in an interdisciplinary project that spans molecular biology, protein biochemistry, chemical kinetics, and spectroscopy.
普渡大学化学系的Mahdi Abu-Omar博士得到化学系无机、生物无机和有机化学项目的支持,研究苯丙氨酸羟化酶(PAH)的活性和底物识别,一种单核非血红素铁羟化酶。一氧化氮与亚铁PAH的反应将用于光谱模型和探测催化相关的铁(II)在非血红素羟化酶中O2结合和活化的早期阶段的配位环境。将确定酶与一氧化氮的四元复合物的分子结构。滴涂沉积拉曼光谱将用于表征酶以及结合到活性位点后的底物中的分子变化。将研究重要酶苯丙氨酸羟化酶的功能,该酶合成必需氨基酸酪氨酸,酪氨酸进一步代谢为重要的神经递质。这项工作将集中在氧是如何在其含铁的活性位点被激活。博士后,研究生和本科生将接受跨学科项目的教育,涵盖分子生物学,蛋白质生物化学,化学动力学和光谱学。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Mahdi Abu-Omar其他文献
Mahdi Abu-Omar的其他文献
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{{ truncateString('Mahdi Abu-Omar', 18)}}的其他基金
CAS: Catalytic funneling and upgrading of biomass-derived phenols and polyols
CAS:生物质衍生酚和多元醇的催化漏斗和升级
- 批准号:
2154333 - 财政年份:2022
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$ 44万 - 项目类别:
Standard Grant
Catalytic Oxidations with Lignin-Based Ligands
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1856753 - 财政年份:2019
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$ 44万 - 项目类别:
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Bioinspired and Sustainable Catalysis for Chlorite Conversion and Dioxygen Reduction
亚氯酸盐转化和分子氧还原的仿生和可持续催化
- 批准号:
1646790 - 财政年份:2016
- 资助金额:
$ 44万 - 项目类别:
Standard Grant
I-Corps: Sustainable manufacturing of natural vanillin
I-Corps:天然香兰素的可持续生产
- 批准号:
1640315 - 财政年份:2016
- 资助金额:
$ 44万 - 项目类别:
Standard Grant
Bioinspired and Sustainable Catalysis for Chlorite Conversion and Dioxygen Reduction
亚氯酸盐转化和分子氧还原的仿生和可持续催化
- 批准号:
1463900 - 财政年份:2015
- 资助金额:
$ 44万 - 项目类别:
Standard Grant
High-Valent Imido and Oxo Complexes in Electron Transfer, Atom Transfer, and Chlorite Catalysis
电子转移、原子转移和亚氯酸盐催化中的高价亚氨基和氧配合物
- 批准号:
1110475 - 财政年份:2011
- 资助金额:
$ 44万 - 项目类别:
Continuing Grant
Mechanistic Studies of Oxygen Evolution and Hydrogen Atom Transfer in Enzymes and Biomimatic Models
酶和仿生模型中析氧和氢原子转移的机理研究
- 批准号:
0749572 - 财政年份:2008
- 资助金额:
$ 44万 - 项目类别:
Continuing Grant
Probing the Mechanism and Structure-Function Relations in Phenylalanine Hydroxylase
苯丙氨酸羟化酶的作用机制及构效关系探讨
- 批准号:
0434637 - 财政年份:2004
- 资助金额:
$ 44万 - 项目类别:
Continuing Grant
Probing the Mechanism and Structure-Function Relations in Phenylalanine Hydroxylase
苯丙氨酸羟化酶的作用机制及构效关系探讨
- 批准号:
0208682 - 财政年份:2002
- 资助金额:
$ 44万 - 项目类别:
Continuing Grant
CAREER: Molecular Rhenium(V) Oxotransferases
职业:分子铼(V) 氧化转移酶
- 批准号:
9874857 - 财政年份:1999
- 资助金额:
$ 44万 - 项目类别:
Continuing Grant
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