In vivo- and in vitro-investigations regarding the impact of hypoxia on ocular tumors (retinoblastoma and melanoma) in correlation to tumor cell motility.

关于缺氧对眼部肿瘤（视网膜母细胞瘤和黑色素瘤）与肿瘤细胞运动相关的影响的体内和体外研究。

• 批准号: 171971315
• 负责人: Privatdozentin Dr. Martina Herwig-Carl
• 金额: --
• 依托单位: Department of Ophthalmology
• 依托单位国家: 德国
• 项目类别: Research Fellowships
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/171971315?language=en
• 关键词: vivo; vitro; investigations; regarding; impact

Hypoxia is one of the major factors regulating the growth behavior of tumors. However, themetabolic conditions in a tumor as well as in its environment are heterogeneous. In ourstudy, the impact of hypoxia on tumor cell motility shall be investigated with the long-termprospective to develop new therapeutic strategies. In vitro- and in vivo-models are used tostudy choroidal melanoma with regard to tumor cell behaviour (especially motility) underdifferent hypoxic conditions. For the in vivo-experiment there is a mouse model allowingstudies in hypoxic (anterior chamber) and normoxic (corneal pocket) conditions, dependingon the injection site of tumor cells. Eventually, computational analysis is used for thecorrelation of time course, cell motility and oxygen levels.

缺氧是调节肿瘤生长行为的主要因素之一。然而，肿瘤及其周围环境中的代谢条件是异质的。本研究将从长期的角度探讨低氧对肿瘤细胞运动的影响，以开发新的治疗策略。体外和体内模型被用来研究脉络膜黑色素瘤在不同缺氧条件下的肿瘤细胞行为（特别是运动性）。对于体内实验，有一种小鼠模型允许在缺氧（前房）和常氧（角膜囊）条件下进行研究，这取决于肿瘤细胞的注射部位。最后，计算分析用于时间过程、细胞运动和氧水平的相关性。