Acquisition of a Spectrofluorometer with Stopped-Flow Kinetics and Fluorescence Life-Time Measurement Capabilities

通过主要研究仪器（MRI）项目的这项奖励，罗斯-霍尔曼理工学院化学系将获得一台具有停止流动动力学和荧光寿命测量能力的荧光光谱仪。该设备将加强以下研究：a)雌激素受体-由配体结合引起的复杂结构和功能变化；b)天然水体中溶解有机物（DOM）的光化学反应性；c)金属离子在DNA氧化损伤中的作用；d)胰岛素聚集过程中流体动力半径的变化。该仪器将把基于激光的技术带入本科教育（包括教学和研究），由此产生的研究将在许多领域产生影响，特别是环境化学和生物化学。

With this award from the Major Research Instrumentation (MRI) Program, the Department of Chemistry at the Rose-Hulman Institute of Technology will acquire a spectrofluorometer with stopped-flow kinetics and fluorescence lifetime measurement capabilities. This equipment will enhance research in investigations on a) the complex structural and functional changes in the estrogen receptor-a induced by ligand binding; b) the photochemical reactivity of dissolved organic matter (DOM) in natural water; c) the role of metal ions in oxidative DNA damage; and d) the change in the hydrodynamic radius of insulin during aggregation. This instrumentation will bring laser-based techniques into the undergraduate education (both teaching and research) and the resulting research will have an impact in a number of areas, especially environmental chemistry and biochemistry.