Acquisition of a Spectrofluorometer with Stopped-Flow Kinetics and Fluorescence Life-Time Measurement Capabilities

在获得重大研究仪器(MRI)计划的这一奖项后，Rose-Hulman理工学院化学系将获得一种具有停流动力学和荧光寿命测量能力的光谱荧光仪。该设备将加强以下方面的研究：a)配体结合引起的雌激素受体-a的复杂结构和功能变化；b)天然水中溶解有机物(DOM)的光化学反应；c)金属离子在DNA氧化损伤中的作用；以及d)胰岛素在聚集过程中流体动力学半径的变化。这种仪器将把基于激光的技术带入本科教育(教学和研究)，由此产生的研究将在许多领域产生影响，特别是环境化学和生物化学。

With this award from the Major Research Instrumentation (MRI) Program, the Department of Chemistry at the Rose-Hulman Institute of Technology will acquire a spectrofluorometer with stopped-flow kinetics and fluorescence lifetime measurement capabilities. This equipment will enhance research in investigations on a) the complex structural and functional changes in the estrogen receptor-a induced by ligand binding; b) the photochemical reactivity of dissolved organic matter (DOM) in natural water; c) the role of metal ions in oxidative DNA damage; and d) the change in the hydrodynamic radius of insulin during aggregation. This instrumentation will bring laser-based techniques into the undergraduate education (both teaching and research) and the resulting research will have an impact in a number of areas, especially environmental chemistry and biochemistry.