Acquisition of a Spectrofluorometer with Stopped-Flow Kinetics and Fluorescence Life-Time Measurement Capabilities

获得具有停流动力学和荧光寿命测量功能的分光荧光计

• 批准号: 0521430
• 负责人: Mark Brandt
• 金额: --
• 依托单位: Rose-Hulman Institute of Technology
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0521430&HistoricalAwards=false
• 关键词: Acquisition; Spectrofluorometer; Stopped; Flow; Kinetics

With this award from the Major Research Instrumentation (MRI) Program, the Department of Chemistry at the Rose-Hulman Institute of Technology will acquire a spectrofluorometer with stopped-flow kinetics and fluorescence lifetime measurement capabilities. This equipment will enhance research in investigations on a) the complex structural and functional changes in the estrogen receptor-a induced by ligand binding; b) the photochemical reactivity of dissolved organic matter (DOM) in natural water; c) the role of metal ions in oxidative DNA damage; and d) the change in the hydrodynamic radius of insulin during aggregation. This instrumentation will bring laser-based techniques into the undergraduate education (both teaching and research) and the resulting research will have an impact in a number of areas, especially environmental chemistry and biochemistry.

凭借主要研究仪器（MRI）计划的这一奖项，Rose-Hulman理工学院的化学系将获得具有停流动力学和荧光寿命测量能力的分光荧光计。这一设备将加强对以下方面的研究：a）配体结合引起的雌激素受体-a的复杂结构和功能变化; B）天然水中溶解有机物的光化学反应性; c）金属离子在氧化DNA损伤中的作用; d）胰岛素在聚集过程中流体动力学半径的变化。这种仪器将把基于激光的技术带入本科教育（教学和研究），由此产生的研究将在许多领域产生影响，特别是环境化学和生物化学。