Research spectrofluorometer and microplate readuer

研究型荧光分光光度计和酶标仪

• 批准号: 345736-2007
• 负责人: Hijri, Mohamed
• 金额: $ 4.36万
• 依托单位: Université de Montréal
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments - Category 1 (<$150,000)
• 财政年份: 2006
• 资助国家: 加拿大
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=326086
• 关键词: Research; spectrofluorometer; microplate; readuer

Fluorescence illumination and measurement is the most rapidly expanding technique employed today, both in the medical and biological sciences, a fact which has spurred the development of more sophisticated fluorescence instrumentation and numerous fluorescent dyes. Spectrofluorometry has now become the method of choice in many applications in modern biology to measure the average fluorescence in bulk samples. This method is commonly used for nucleic acids (DNA and RNA) and protein quantitation and purity, ELISA, enzyme kinetics, calcium, pH, chloride, sodium, potassium and other ions that are measurable by fluorescent dyes. The availability of living colors fluorescent proteins such as the GFP and reef coral fluorescent proteins, and the ease of conjugating fluorescent dyes to proteins, investigations into the functionality of molecules and proteins has become increasingly accessible. Although these techniques are now accessible to most laboratories in the Institute because of its modern laboratories and equipments. Currently, one fluorescent microplate reader is available at the institute. However, this instrument uses a limited number of filter sets for excitation and emission, which is not accurate for many applications such as enzyme kinetics and PicoGreen DNA detection. In addition, whenever a new fluorescence dye comes out we need to buy expensive filters. If granted, the expanded availability of the scanning specrtofluorometer opens the door not only to new researchers and students, but also to new applications such as Endpoint, kinetic and spectrum. The many different projects described in the proposal illustrate the diversity of new projects, which will become feasible with new equipment, and demonstrate that the new equipment will be extensively utilized.

荧光照明和测量是当今在医学和生物科学中应用最迅速的技术，这一事实刺激了更复杂的荧光仪器和许多荧光染料的发展。在现代生物学的许多应用中，荧光光谱法已成为测量大量样品中平均荧光的首选方法。该方法常用于核酸（DNA和RNA）和蛋白质的定量及纯度、ELISA、酶动力学、钙、pH、氯、钠、钾等荧光染料可测量的离子。活体彩色荧光蛋白（如GFP和珊瑚礁珊瑚荧光蛋白）的可用性，以及荧光染料与蛋白质结合的便利性，对分子和蛋白质功能的研究变得越来越容易。尽管由于拥有现代化的实验室和设备，研究所的大多数实验室现在都可以使用这些技术。目前，该研究所有一台荧光微孔板阅读器。然而，该仪器使用有限数量的过滤器集的激发和发射，这是不准确的许多应用，如酶动力学和PicoGreen DNA检测。此外，每当一种新的荧光染料问世时，我们都需要购买昂贵的过滤器。如果获得批准，扫描光谱荧光仪的扩展可用性不仅为新的研究人员和学生打开了大门，而且还为端点，动力学和光谱等新应用打开了大门。提案中描述的许多不同的项目说明了新项目的多样性，新设备将成为可行的，并证明新设备将被广泛利用。