SBIR Phase I: Biomolecule Immobilization Chemistry for Nanomagnetic/Spintronic Biosensor Array

SBIR 第一阶段：纳米磁性/自旋电子生物传感器阵列的生物分子固定化化学

• 批准号: 0539834
• 负责人: Patrick Guire
• 金额: $ 9.96万
• 依托单位: Innovative Surface Technologies, Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-01-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0539834&HistoricalAwards=false
• 关键词: SBIR; Phase; Biomolecule; Immobilization; Chemistry

This Small Business Innovation Research (SBIR) Phase I project is designed to adapt a Giant MagnetoResistive (GMR) sensor device into a "spintronic" multianalyte biosensor. The potential value of biosensor technology is being recognized for a wide range of applications, from medical diagnostics to countering bio-terrorism. Magnetic nano/microparticles present advantages as labels for biomolecule binding, including stability and low background signal. This project will provide improved biomolecule coupling technology for biosensor applications of certain GMRmicroelectrode array and the paramagnetic microbeads under development. The Phase I effort will include the synthesis and use testing of photochemical and electrochemical polymer derivatives for binding oligonucleotides to the silicon nitride surface of GMR electrodes and to uncoated NiFe microparticles. Biomolecule binding activity, stability, and signal/noise properties will be measured by fluorescent and magnetic assay methods and shown to be equal or superior to those of currently used assays. These Phase I results are expected to provide a solid technical background for optimizing the biomolecule immobilization chemistry in Phase II, as well as new but related latent-reactive polymers for enhanced microfluidic channel coating and lid adhesion for the GMR biosensor flow cell.Commercially coating reagents and procedures for specific biomolecule binding on microelectrode arrays and on paramagnetic metal nano/microparticles are expected to command a significant fraction of the greater than $5 billion genomic and proteomic assay disposables market expected by 2010. They have the capacity to give greatly lowered cost and enhanced ease of use for consumers in the military, personal care, and security fields. The coatings will help enable a lab-on-a-chip sensor that can be used in the field to give accurate and timely readouts on biohazards, potentially saving people's lives. Additionally, the development of a new general coating technology for magnetic materials will have impacts beyond biosensors, including ferrofluids, magnetic memory devices, and many others.

这个小型企业创新研究(SBIR)第一阶段项目旨在将巨磁阻(GMR)传感器设备改造成“自旋电子式”多分析物生物传感器。从医学诊断到打击生物恐怖主义，生物传感器技术的潜在价值正在被广泛的应用所认识。磁性纳米/微米颗粒作为生物分子结合的标记物具有稳定性好、背景信号低等优点。该项目将为某些GMR微电极阵列和正在开发的顺磁微球的生物传感器应用提供改进的生物分子耦合技术。第一阶段的工作将包括光化学和电化学聚合物衍生物的合成和使用测试，用于将寡核苷酸结合到GMR电极的氮化硅表面和未涂覆的NiFe微粒上。生物分子结合活性、稳定性和信号/噪声特性将通过荧光和磁性分析方法进行测量，并显示出与当前使用的分析方法相同或更好的结果。这些第一阶段的结果预计将为优化第二阶段的生物分子固定化学提供坚实的技术背景，以及用于增强GMR生物传感器流动细胞的微流控通道涂层和盖子粘附性的新型但相关的潜在反应聚合物。商业涂层试剂和方法用于特定生物分子在微电极阵列和顺磁性金属纳米/微粒子上的结合，预计将在2010年超过50亿美元的基因组和蛋白质组分析一次性市场中占据相当大的份额。它们有能力在军事、个人护理和安全领域为消费者提供极大的成本和更高的易用性。这种涂层将有助于实现单芯片实验室传感器，该传感器可以在现场使用，提供准确和及时的生物危害读数，有可能拯救人们的生命。此外，一种新的磁性材料通用涂层技术的发展将产生超越生物传感器的影响，包括磁流体、磁记忆器件等。