Semiconductor Surface Chemistry for Biosensors

光亲和固定特定结合蛋白的概念已获得专利，并减少了该公司在玻璃上的抗体实践。提出了用于受体分子（特别是抗体）与钝化（亲水性间隔涂层）硅基传感器活化表面的亲和偶联（共价）的其他试剂和反应程序的开发。额外的三功能光反应试剂将被合成并偶联到传感器表面，用于单克隆抗体的特异性共价结合（使用两个抗体结合位点中的一个），留下剩余的稳定抗体结合位点，用于测试样品中表面固定分析物和自由分析物分子之间的功能交换。特异性的一步纯化-通过靠近短间隔固定分析物基团的长，亲水，柔性系绳将抗体共价固定，有望提供具有更高性能（更快的响应和再生，以及更高的灵敏度）的稳定免疫传感器的成本效益生产。特别是在需要快速分析的地方)。该系统将检测甲苯和/或相关的芳香烃衍生物。用这种固定的、稳定的免疫传感器系统和这种类型的分析物证明实验室的可行性，将支持该技术对生物活性物质的敏感、快速和选择性传感和监测的实用性。该传感器装置将在批量或连续取样情况下可逆地识别和定量分析物含量，并通过电子记录和数据传输信号。这种装置将提供广泛的适用性，用于定期样品分析和实时在线监测流体（空气、水）以及空气和生理流体中的剂量测量。

The concept of photoaffinity immobilization of specific binding proteins has been patented and reduced to practice with antibody on glass by this company. The development of additional reagents and reaction procedures is proposed for the affinity coupling (covalent) of receptor molecules (especially antibodies) to the activated surface of passivated (by hydrophilic spacer coating) silicon-based sensors. Additional trifunctional photoreactive reagents will be synthesized and coupled to the sensor surface for the specific covalent binding of monoclonal antibody (using one of the two antibody binding sites), leaving the remaining stabilized antibody binding site available for functional exchange between surface-immobilized analyte and free analyte molecules in the test sample. The specific, one-step purification-covalent immobilization of the antibody through a long, hydrophilic, flexible tether adjacent to the short-spacer immobilized analyte group, is expected to provide cost efficient production of stabilized immunosensors with improved performance (faster response and regeneration, as well as increased sensitivity, particularly where rapid analyses are required). The system proposed will detect toluene and/or related aromatic hydrocarbon derivatives. Demonstrating laboratory feasibility with this immobilized, stabilized immunosensor system with this type of analyte will support the utility of this technology for sensitive, rapid, and selective sensing and monitoring of biologically active agents. The sensor device will reversibly recognize and quantitate analyte content in batch or continuous sampling situations and transduce the signal with electronic recording and transmission of data. Such a device would offer a broad range of applicability for periodic sample analysis and real time, on line monitoring of fluids (air, water) as well as dosimetry measurements in air and physiological fluids.