Semiconductor Surface Chemistry for Biosensors

用于生物传感器的半导体表面化学

• 批准号: 9060358
• 负责人: Patrick Guire
• 金额: $ 5万
• 依托单位: SurModics, Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-01-01 至 1991-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9060358&HistoricalAwards=false
• 关键词: Semiconductor; Surface; Chemistry; Biosensors

The concept of photoaffinity immobilization of specific binding proteins has been patented and reduced to practice with antibody on glass by this company. The development of additional reagents and reaction procedures is proposed for the affinity coupling (covalent) of receptor molecules (especially antibodies) to the activated surface of passivated (by hydrophilic spacer coating) silicon-based sensors. Additional trifunctional photoreactive reagents will be synthesized and coupled to the sensor surface for the specific covalent binding of monoclonal antibody (using one of the two antibody binding sites), leaving the remaining stabilized antibody binding site available for functional exchange between surface-immobilized analyte and free analyte molecules in the test sample. The specific, one-step purification-covalent immobilization of the antibody through a long, hydrophilic, flexible tether adjacent to the short-spacer immobilized analyte group, is expected to provide cost efficient production of stabilized immunosensors with improved performance (faster response and regeneration, as well as increased sensitivity, particularly where rapid analyses are required). The system proposed will detect toluene and/or related aromatic hydrocarbon derivatives. Demonstrating laboratory feasibility with this immobilized, stabilized immunosensor system with this type of analyte will support the utility of this technology for sensitive, rapid, and selective sensing and monitoring of biologically active agents. The sensor device will reversibly recognize and quantitate analyte content in batch or continuous sampling situations and transduce the signal with electronic recording and transmission of data. Such a device would offer a broad range of applicability for periodic sample analysis and real time, on line monitoring of fluids (air, water) as well as dosimetry measurements in air and physiological fluids.

特异性结合蛋白的光亲和固定化概念已获得专利，并由该公司通过玻璃上的抗体进行实践。建议开发额外的试剂和反应程序，以将受体分子（尤其是抗体）亲和耦合（共价）到钝化（通过亲水间隔涂层）硅基传感器的活化表面。额外的三功能光反应试剂将被合成并耦合到传感器表面，用于单克隆抗体的特异性共价结合（使用两个抗体结合位点之一），留下剩余的稳定抗体结合位点可用于测试样品中表面固定的分析物和游离分析物分子之间的功能交换。通过与短间隔基固定分析物基团相邻的长的、亲水的、柔性的系链对抗体进行特异性的一步纯化共价固定，预计将提供具有改进性能（更快的响应和再生，以及更高的灵敏度，特别是在需要快速分析的情况下）的稳定免疫传感器的成本效益生产。所提出的系统将检测甲苯和/或相关的芳香烃衍生物。利用这种具有此类分析物的固定、稳定的免疫传感器系统来证明实验室的可行性，将支持该技术在生物活性剂的灵敏、快速和选择性传感和监测中的应用。传感器装置将在批量或连续采样情况下可逆地识别和定量分析物含量，并通过电子记录和数据传输来转换信号。这种设备将为定期样品分析和液体（空气、水）的实时在线监测以及空气和生理液体中的剂量测定提供广泛的适用性。