Semiconductor Surface Chemistry for Biosensors

光亲和固定化特定结合蛋白的概念已经获得专利，并被该公司简化为在玻璃上使用抗体。建议开发额外的试剂和反应程序，使受体分子(特别是抗体)与钝化(通过亲水间隔涂层)的硅基传感器的活化表面亲和偶联(共价)。还将合成额外的三官能团光反应试剂，并将其偶联到传感器表面，以与单抗进行特定的共价结合(使用两个抗体结合位点之一)，使剩余的稳定抗体结合位点可用于测试样品中表面固定化分析物和游离分析物分子之间的功能交换。通过与短间隔基固定化分析物基团相邻的长的、亲水的、灵活的系链对抗体进行特定的一步纯化-共价固定化抗体，预计将提供成本效益高的稳定免疫传感器的生产，并改善性能(更快的响应和再生，以及更高的灵敏度，特别是在需要快速分析的情况下)。建议的系统将检测甲苯和/或相关的芳香烃衍生物。用这种类型的分析物证明这种固定化、稳定的免疫传感器系统在实验室的可行性，将支持这项技术用于敏感、快速和选择性的生物活性物质的传感和监测。传感器设备将在批量或连续采样的情况下可逆地识别和定量分析物含量，并通过电子记录和数据传输来转换信号。这种装置将为定期样品分析和流体(空气、水)的实时在线监测以及空气和生理液体中的剂量测量提供广泛的适用性。

The concept of photoaffinity immobilization of specific binding proteins has been patented and reduced to practice with antibody on glass by this company. The development of additional reagents and reaction procedures is proposed for the affinity coupling (covalent) of receptor molecules (especially antibodies) to the activated surface of passivated (by hydrophilic spacer coating) silicon-based sensors. Additional trifunctional photoreactive reagents will be synthesized and coupled to the sensor surface for the specific covalent binding of monoclonal antibody (using one of the two antibody binding sites), leaving the remaining stabilized antibody binding site available for functional exchange between surface-immobilized analyte and free analyte molecules in the test sample. The specific, one-step purification-covalent immobilization of the antibody through a long, hydrophilic, flexible tether adjacent to the short-spacer immobilized analyte group, is expected to provide cost efficient production of stabilized immunosensors with improved performance (faster response and regeneration, as well as increased sensitivity, particularly where rapid analyses are required). The system proposed will detect toluene and/or related aromatic hydrocarbon derivatives. Demonstrating laboratory feasibility with this immobilized, stabilized immunosensor system with this type of analyte will support the utility of this technology for sensitive, rapid, and selective sensing and monitoring of biologically active agents. The sensor device will reversibly recognize and quantitate analyte content in batch or continuous sampling situations and transduce the signal with electronic recording and transmission of data. Such a device would offer a broad range of applicability for periodic sample analysis and real time, on line monitoring of fluids (air, water) as well as dosimetry measurements in air and physiological fluids.