Semiconductor Surface Chemistry for Biosensors

特异性结合的光亲和固定化概念 蛋白质已经获得专利，并减少到与抗体的实践 在玻璃上，这家公司。其他试剂的开发 提出了亲和偶联的反应步骤 受体分子（特别是抗体）与受体分子（特别是抗体）的共价结合。 钝化的活化表面（通过亲水性间隔涂层） 硅基传感器额外的三官能光反应性 试剂将被合成并耦合到传感器表面 对于单克隆抗体的特异性共价结合（使用 两个抗体结合位点之一），留下剩余的 稳定的抗体结合位点可用于功能性 表面固定分析物和游离分析物之间的交换 测试样品中的分子。具体来说，一步 纯化-通过共价键固定抗体， 与短间隔物相邻的长的亲水性柔性系链 固定的分析物基团，预计将提供成本效益 具有改进性能的稳定的免疫传感器的生产 （更快的反应和再生，以及增加 敏感性，特别是在需要快速分析的情况下）。的 所提出的系统将检测甲苯和/或相关芳族化合物 烃衍生物。实验室可行性论证 用这个固定的，稳定的免疫传感器系统， 分析物的类型将支持该技术的实用性， 灵敏、快速和选择性地感知和监测 生物活性剂。传感器装置将可逆地 批量或连续识别和定量分析物含量 采样情况并将信号与电子 数据的记录和传输。这样的设备将提供一个 广泛适用于定期样品分析， 真实的实时、在线监测流体（空气、水）以及 空气和生理液体中的剂量测定。

The concept of photoaffinity immobilization of specific binding proteins has been patented and reduced to practice with antibody on glass by this company. The development of additional reagents and reaction procedures is proposed for the affinity coupling (covalent) of receptor molecules (especially antibodies) to the activated surface of passivated (by hydrophilic spacer coating) silicon-based sensors. Additional trifunctional photoreactive reagents will be synthesized and coupled to the sensor surface for the specific covalent binding of monoclonal antibody (using one of the two antibody binding sites), leaving the remaining stabilized antibody binding site available for functional exchange between surface-immobilized analyte and free analyte molecules in the test sample. The specific, one-step purification-covalent immobilization of the antibody through a long, hydrophilic, flexible tether adjacent to the short-spacer immobilized analyte group, is expected to provide cost efficient production of stabilized immunosensors with improved performance (faster response and regeneration, as well as increased sensitivity, particularly where rapid analyses are required). The system proposed will detect toluene and/or related aromatic hydrocarbon derivatives. Demonstrating laboratory feasibility with this immobilized, stabilized immunosensor system with this type of analyte will support the utility of this technology for sensitive, rapid, and selective sensing and monitoring of biologically active agents. The sensor device will reversibly recognize and quantitate analyte content in batch or continuous sampling situations and transduce the signal with electronic recording and transmission of data. Such a device would offer a broad range of applicability for periodic sample analysis and real time, on line monitoring of fluids (air, water) as well as dosimetry measurements in air and physiological fluids.