SBIR Phase I: Binding Protein Patterns on Plastic Surfaces

* 9661439 Guire 该项目旨在证明一种普遍适用的，简便的，具有成本效益的方法来涂覆用于特异性结合测定和生物传感器系统的塑料材料的可行性。共价钝化的表面与生物分子相容的稳定剂，图案化的生物分子的固定，这是关键的和持久的障碍，生物传感器技术的商业发展，将通过制备光反应性的“通用结合”薄膜表面解决。高亲和力通用配体（生物素）衍生物和钝化/稳定剂的共价光化学键合将分别通过图案化和均匀照明在温和的反应条件下快速完成。固定在钝化光学级塑料上的X-抗生物素蛋白的图案化阵列将通过用该多位点结合蛋白的粗品或纯化溶液浸涂该表面来获得，然后制备用于基本上任何核酸探针或特异性分析物结合蛋白（例如，抗体）测定（例如，具有用于囊性纤维化基因缺陷的所需生物素化寡核苷酸）。 这种方法预计将提供具有成本效益的解决方案，以突出的技术问题的小样本，敏感，multiananyte，坚固耐用的便携式商业设备正在开发的核酸探针和免疫测定的价值，环境，食品，传染病，基因序列分析和监测的生物分子传感器接口。 ***

*** 9661439 Guire This project is designed to demonstrate the feasibility of a generally applicable, facile, and cost-effective approach to coating of plastic materials used in specific binding assay and biosensor systems. Covalent passivation of the surface with a biomolecule-compatible stabilizing agent, patterned immobilization of biomolecules, which are critical and enduring obstacles to the commercial development of biosensor technology, will be resolved through the preparation of a photoreactive "universal binding" thin film surface. Covalent photochemical bonding of the high affinity general ligand (biotin) derivative and of the passivating/stabilizing agent, will be accomplished quickly under mild reaction conditions by patterned and uniform illumination, respectively. A patterned array of X-avidin immobilized on passivated optical grade plastic will be obtained by dip-coating this surface with a crude or purified solution of this multi-site binding protein, then prepared for multianalyte assay use with essentially any nucleic acid probes or specific analyte binding proteins (e.g., antibodies) assay through differential loading of the individual assay dots of the array by inkjet printing (e.g., with the desired biotinylated oligonucleotides for cystic fibrosis gene defects). This approach is expected to provide cost-effective solutions to the outstanding technical problems of the biomolecule-sensor interface of small sample, sensitive , multiananyte, rugged portable commercial devices under development for nucleic acid probe and immune assays of value for environmental, food, infectious disease, and gene sequence analysis and monitoring. ***