SGER: Single Molecule-Radiation Interaction in Whispering-Gallery Mode Evanescent Field

生物科学基金会摘要提案编号：CTS-0541585主要研究者：郭志雄单位： 罗格斯大学提案标题： SGER：回音壁模式瞬逝场中单分子-辐射相互作用该提案是作为探索性研究（SGER）类别的小型赠款中化学和传输系统部门的主动提交，随后被分配到热系统计划的热传输和热处理子元素。 本项目的目标是了解单分子与倏逝辐射场的相互作用如何影响回音壁模式（WGM）微共振，以便基于WGM的光学传感器可以作为分子探测的新工具进行探索。 该项目的目标将是模拟使用拟议的WGM微谐振传感器检测单个蛋白质和蛋白质-蛋白质相互作用。纳米纤维的最新进展使得在具有微米/纳米级物理尺寸的精细且均匀的微腔中考虑光学谐振成为可能。这种器件能够实现各种超灵敏和紧凑集成的传感器技术。该探索性项目将研究和证明使用WGM微传感器无标记检测单个生物分子和分子相互作用的可行性，能力和灵敏度。如果成功的话，这项探索性的工作将带来进步，可能导致开发出灵敏、选择性和稳定的实用设备，并具有快速响应时间。 除了蛋白质之外，可以固定在WGM传感器上的配体的其他实例包括用于感测特定抗原的存在的抗体、用于通过双链化捕获的DNA、或用于检测特定底物/配体的酶/受体。在食品安全分析和检测低水平化学品和毒素方面的潜在应用将有助于国家打击生物恐怖主义的总体战略。 这项研究将影响研究生和本科生谁将参与该项目的教育。学生参与研究为他们提供了机会，扩大他们的知识视野，跨学科研究，促进工程专业学生的培训，为高科技劳动力做好准备。研究成果将在专业期刊、国家和国际会议上广泛传播，造福社区和整个社会。

NATIONAL SCIENCE FOUNDATIONABSTRACTProposal Number: CTS-0541585Principal Investigator: Guo, ZhixiongAffiliation: Rutgers UniversityProposal Title: SGER: Single Molecule-Radiation Interaction in Whispering Gallery Mode Evanescent Field The proposal was received as an unsolicited submission to the Chemical and Transport Systems Division in the Small Grants for Exploratory Research (SGER) category and was subsequently assigned to the Thermal Transport and Thermal Processing Sub-element of the Thermal Systems Program. The objective of this project is to understand how the interactions of single molecules with the evanescent radiation field affect the whispering-gallery mode (WGM) microresonances such that WGM-based optical sensors can be explored as a new tool for molecular probing. The project objectives will be to simulate the detection of single proteins and protein-protein interactions using the proposed WGM microresonant sensor. Recent advances in nanofabrication have made it feasible to consider optical resonances in fine and uniform microcavities having physical dimensions at the micro/nanometer levels. Such devices enable a wide array of ultra-sensitive and compactly integrated sensor technologies. This exploratory project will examine and demonstrate the feasibility, capability and sensitivity of label-free detection of single biomolecules and molecular interactions using WGM microsensors. If successful, this exploratory work would lead to advances that may lead to the development of practical devices that are sensitive, selective, and stable with rapid response time. In addition to proteins, other examples of ligands that can be immobilized on the WGM sensor include antibodies to sense the presence of specific antigens, DNA for capture through hydridization, or enzymes/receptors for the detection of specific substrates/ligands. The potential applications to food safety analysis and detection of low levels of chemicals and toxins would contribute to the nation's general strategy in combating bio-terrorism. The research will impact the education of graduate students and undergraduate students who will participate in the project. The participation of students in research offers them the opportunities to expand their intellectual horizon to interdisciplinary research and promotes the training of engineering students to prepare for the high-technology workforce. The research results will be widely disseminated in professional journals, and national and international conferences and benefit the community and society at large.