A High Throughput Protoplast System for Rice Functional Genomics and Proteomics: Protein-Protein Interactions at the Host-Pathogen Interface
用于水稻功能基因组学和蛋白质组学的高通量原生质体系统:宿主-病原体界面的蛋白质-蛋白质相互作用
基本信息
- 批准号:0605017
- 负责人:
- 金额:$ 234.53万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2006
- 资助国家:美国
- 起止时间:2006-06-15 至 2013-05-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
A High Throughput Protoplast System for Rice Functional Genomics and Proteomics: Protein-Protein Interactions at the Host-Pathogen InterfaceRice is the food staple for over half the world's population. Rice blast disease, caused by the fungal pathogen Magnaporthe grisea, is highly devastating and persists as a major threat to global food security. The ability of host and pathogen to detect and respond to each other's presence is governed by protein interactions, however knowledge of these interactions remains fragmentary at best. Access to near complete genomes of both rice and M. grisea, along with the extensive biological resources and a rich history of investigation, provides the opportunity to investigate interactions between host and pathogen. This research project will develop and test a novel system based on the reassembly of dissected fragments of light/fluorescence generating proteins fused to associating fungal and/or rice peptides to directly visualize protein interactions in living plant cells. Rice protoplasts (cells devoid of their cell wall) will be using since they are easy to manipulate, are amenable to large-scale analyses and typically mimic whole plants. As a proof-of-concept, the system will be first tested using rice proteins that are known to interact. The applicability of the system to interrogate the interaction of proteins from rice with those of the rice blast fungus will be subsequently tested using a known defense elicitor (avirulence) protein and its cognate rice resistance receptor. The system will be finally scaled up to screen for unknown fungal and plant protein associations that are predicted to control the deployment of host defenses. The high throughput protoplast system will be also applied in a feasibility study to screen for unknown fungal elicitors of plant host defense responses, of which a select few will advance into the novel screen developed to identify interacting plant proteins. An extensive education plan for high school students and activities promoting recruitment and participation by members of underrepresented groups are planned. In addition to furthering our basic understanding of plant-pathogen interactions, the technologies to be developed will have far-reaching utility for the discovery and evaluation of other protein-protein or protein-ligand interactions in plants systems outside rice and rice blast. Data from this project will be made available in refereed publications. Data, protocols, and reagents will also be made avaiable via www.fsgc.net (Fungal Genetics Stock Center) and www.mgosdb.org (Magnaportha grisea Oriza sativa database) as they are generated and qualities checked.
用于水稻功能基因组学和蛋白质组学的高通量原生质体系统:宿主-病原体界面的蛋白质-蛋白质相互作用水稻是世界一半以上人口的主食。 稻瘟病由真菌病原体稻瘟病引起,具有高度破坏性,一直是全球粮食安全的主要威胁。宿主和病原体检测和响应彼此存在的能力是由蛋白质相互作用控制的,但是对这些相互作用的了解充其量仍然是零散的。 获得水稻和稻瘟病菌近乎完整的基因组,以及广泛的生物资源和丰富的研究历史,为研究宿主和病原体之间的相互作用提供了机会。 该研究项目将开发和测试一种新的系统,该系统基于光/荧光产生蛋白的解剖片段的重新组装,该蛋白与真菌和/或水稻肽融合,以直接可视化活植物细胞中的蛋白质相互作用。 将使用水稻原生质体(没有细胞壁的细胞),因为它们易于操作,适合大规模分析并且通常模仿整个植物。作为概念验证,该系统将首先使用已知相互作用的大米蛋白进行测试。 随后将使用已知的防御激发子(无毒)蛋白及其同源水稻抗性受体来测试该系统询问水稻蛋白质与稻瘟菌蛋白质相互作用的适用性。 该系统最终将扩大规模,以筛选未知的真菌和植物蛋白关联,这些蛋白关联预计将控制宿主防御的部署。高通量原生质体系统还将应用于可行性研究,以筛选植物宿主防御反应的未知真菌激发子,其中少数将进入为鉴定相互作用的植物蛋白而开发的新型筛选。计划为高中生制定一项广泛的教育计划,并开展促进代表性不足群体成员招募和参与的活动。除了进一步加深我们对植物与病原体相互作用的基本了解外,待开发的技术还将对发现和评估水稻和稻瘟病以外的植物系统中其他蛋白质-蛋白质或蛋白质-配体相互作用具有深远的用途。该项目的数据将在参考出版物中提供。 数据、方案和试剂在生成和质量检查时也将通过 www.fsgc.net(真菌遗传学库存中心)和 www.mgosdb.org(Magnaportha grisea Oriza sativa 数据库)提供。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Ralph Dean其他文献
Fungal biology reaps the benefit of genomics
- DOI:
10.1186/gb-2005-6-8-336 - 发表时间:
2005-01-01 - 期刊:
- 影响因子:9.400
- 作者:
Ralph Dean;Thomas Mitchell;Nicole Donofrio;Jun-Seop Jeong;Amy Powell - 通讯作者:
Amy Powell
Ralph Dean的其他文献
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{{ truncateString('Ralph Dean', 18)}}的其他基金
Global Proteome and Signal Pathway Phosphoproteome Dynamics during Appressorium Formation in the Rice Blast Fungus Magnaporthe oryzae
稻瘟病菌 Magnaporthe oryzae 附着胞形成过程中的整体蛋白质组和信号通路磷酸化蛋白质组动态
- 批准号:
0918611 - 财政年份:2009
- 资助金额:
$ 234.53万 - 项目类别:
Continuing Grant
Comparative Genome Analysis of Foliar and Root Infecting Members of the Magnaporthaceae
Magnaporthaceae 叶和根感染成员的比较基因组分析
- 批准号:
0731808 - 财政年份:2007
- 资助金额:
$ 234.53万 - 项目类别:
Standard Grant
Microbial Genome Sequencing: Genome Sequence of Magnaporthe Grisea
微生物基因组测序:稻瘟病菌基因组序列
- 批准号:
0136064 - 财政年份:2001
- 资助金额:
$ 234.53万 - 项目类别:
Standard Grant
Whole Genome Analysis of Pathogen-Host Recognition and Subsequent Responses in the Rice Blast Patho-System
稻瘟病致病系统中病原体宿主识别和后续反应的全基因组分析
- 批准号:
0115642 - 财政年份:2001
- 资助金额:
$ 234.53万 - 项目类别:
Cooperative Agreement
Expression and Functional Analysis of Genes Regulating Appressorium Formation in Magnaporthe Grisea
稻瘟病菌附着胞形成调控基因的表达及功能分析
- 批准号:
0096210 - 财政年份:1999
- 资助金额:
$ 234.53万 - 项目类别:
Standard Grant
Expression and Functional Analysis of Genes Regulating Appressorium Formation in Magnaporthe Grisea
稻瘟病菌附着胞形成调控基因的表达及功能分析
- 批准号:
9874430 - 财政年份:1999
- 资助金额:
$ 234.53万 - 项目类别:
Standard Grant
Dissection of Appressorium Formation in Magnaporthe grisea
稻瘟病附着胞形成的解剖
- 批准号:
9513808 - 财政年份:1996
- 资助金额:
$ 234.53万 - 项目类别:
Continuing Grant
Isolation of Genes Mediating Cell-Surface Induction of Infection Structures
介导细胞表面感染结构诱导的基因的分离
- 批准号:
9219561 - 财政年份:1993
- 资助金额:
$ 234.53万 - 项目类别:
Continuing Grant
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