Global Proteome and Signal Pathway Phosphoproteome Dynamics during Appressorium Formation in the Rice Blast Fungus Magnaporthe oryzae

稻瘟病菌 Magnaporthe oryzae 附着胞形成过程中的整体蛋白质组和信号通路磷酸化蛋白质组动态

• 批准号: 0918611
• 负责人: Ralph Dean
• 金额: $ 52.94万
• 依托单位: North Carolina State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-15 至 2016-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0918611&HistoricalAwards=false
• 关键词: Global; Proteome; Signal; Pathway; Phosphoproteome

Plant pathogenic fungi are responsible for major crop losses. To infect their hosts many fungi, including Magnaporthe oryzae, the causal agent of rice blast and the most destructive fungal disease of rice worldwide, have evolved to form a specialized infection cell, an appressorium. Appressorium formation is regulated by signal transduction pathways notably the cAMP and MAP kinase pathways, which results in activation of phosphorylation cascades leading to changes in gene expression, including genes involved in protein turnover. However, knowledge of these post-translational protein modifications and dynamic changes in proteins during appressorium formation is limited. The ability to conduct large scale analyses of proteins in living cells as well as measure changes in protein modifications has advanced dramatically in recent years. In this project, to further define and characterize the processes regulating appressorium formation, a comprehensive quantitative analysis of relative changes in protein abundance and phosphorylation patterns during appressorium formation will be conducted. The integration of gene expression and proteomics data will contribute substantially to constructing a more comprehensive view of the cellular processes underlying this fundamental biological process.Broader Impacts: This project will have broad impact at several levels. The research will provide cross training in chemistry and fungal biology for the graduate student and post doctoral scholar involved in this project. An individualized mentoring program will be developed for the post doctoral scholar. The PI and co-PI will develop a new colloquium integrating general concepts in biology and chemistry around the premises of this project for high school students as well as provide research experience for undergraduates. Finally, through broad dissemination of the results from these studies, including an online open-source database, the knowledge will be invaluable for driving research to find practical solutions to rice blast and other diseases caused by fungal plant pathogens.

植物病原真菌是造成农作物损失的主要原因。为了侵染寄主，许多真菌，包括稻瘟病的病原菌和世界上最具破坏性的水稻真菌病，已经进化成一种特殊的感染细胞--附着胞。附着胞的形成受信号转导通路特别是cAMP和MAP激酶通路的调节，这两个通路导致磷酸化级联反应的激活，从而导致基因表达的变化，包括参与蛋白质周转的基因。然而，关于这些翻译后蛋白质修饰和附着胞形成过程中蛋白质的动态变化的知识是有限的。近年来，对活细胞中的蛋白质进行大规模分析以及测量蛋白质修饰的变化的能力有了显着的进步。在这个项目中，为了进一步确定和表征附着胞形成的调控过程，将对附着胞形成过程中蛋白质丰度和磷酸化模式的相对变化进行全面的定量分析。基因表达和蛋白质组学数据的整合将大大有助于构建对这一基本生物过程背后的细胞过程的更全面的看法。广泛的影响：该项目将在几个层面上产生广泛的影响。这项研究将为参与该项目的研究生和博士后学者提供化学和真菌生物学方面的交叉培训。将为博士后学者制定个性化的指导计划。PI和共同PI将围绕这个项目的前提为高中生开发一个新的研讨会，整合生物和化学的一般概念，并为本科生提供研究经验。最后，通过广泛传播这些研究的结果，包括一个在线开源数据库，这些知识将对推动研究寻找稻瘟病和其他由真菌植物病原体引起的疾病的实际解决方案具有无价的价值。