Whole Genome Analysis of Pathogen-Host Recognition and Subsequent Responses in the Rice Blast Patho-System

稻瘟病致病系统中病原体宿主识别和后续反应的全基因组分析

• 批准号: 0115642
• 负责人: Ralph Dean
• 金额: $ 739.48万
• 依托单位: North Carolina State University
• 依托单位国家: 美国
• 项目类别: Cooperative Agreement
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-10-01 至 2006-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0115642&HistoricalAwards=false
• 关键词: Whole; Genome; Analysis; Pathogen; Host

Rice blast disease, caused by the fungus Magnaporthe grisea, is a leading constraint to rice production and is a serious threat to food security worldwide. The goal of this project is to elucidate the basis of plant resistance through a comprehensive analysis of the molecular events that occur during pathogen-host recognition and the subsequent defense responses. Our ultimate goal is to design durable disease resistance. Rice blast is a compelling experimental system for conducting such analyses. A complete understanding of the interaction requires knowledge of the genes in both pathogen and host. In this project, a set of crucial ESTs involved in rice defense will be identified. This information, along with other data, will be used for functional genomics studies, specifically microarray analysis and gene knockout, to uncover many of the early events in host-pathogen recognition from the perspectives of both the pathogen and host. As a result, major new resources for distribution and long term use by the plant, cell and pathogen biology research communities will be created. To facilitate access to resources and research data, a new database (MGOS) will be developed that will serve as a bridge between plant and pathogen research communities. An interactive educational component "Genomics and Food Security" will be designed and developed for high school students founded on the research. The project includes a major component to promote research experiences for undergraduates from underrepresented groups including the formation of a Genomics Club that will integrate other Plant Genome projects at North Carolina State University.Deliverables:1. 50,000 mutants with corresponding phenotype information, digital photographs, and genomic DNA 2. Identification and cloning of mutated genes shown to affect fungal penetration and pathogenesis3. Putatively secreted proteins, plant responses, and their deduced functions4. Micro-arrays and the results of transcriptional profiling experiments looking at gene regulation in the rice host and the fungal pathogen5. SAGE libraries from infected rice tissue6. 35,000 ESTs from different rice tissues infected and non-infected7. Public database (MGOS) housing all data generated during the course of this project.8. Teaching module "Genomics and food security" for high school teachers and students Contact Information for Deliverables: 1. Project Leader, Thomas Mitchell, North Carolina State University, [e-mail], 2. Project Director, Ralph Dean, North Carolina State University, [e-mail], 3. Bioinformatics, Cari Soderlund, University of Arizona, [e-mail]4. Education and Outreach, Brenda Wojnowski, North Carolina State University, [e-mail] Internet Addresses (Web or Email) for Project Information: 1. http://www.fungalgenomics.ncsu.edu2. http://www.riceblast.org3. [e-mail]4. [e-mail]

由稻瘟病菌（Magnaporthe grisea）引起的稻瘟病是水稻生产的主要制约因素，对全世界的粮食安全构成严重威胁。本项目的目标是通过对病原体-寄主识别过程中发生的分子事件以及随后的防御反应的综合分析来阐明植物抗性的基础。我们的最终目标是设计持久的抗病能力。稻瘟病是进行这种分析的一个引人注目的实验系统。要完全理解这种相互作用，需要了解病原体和宿主中的基因。在这个项目中，一组关键的EST参与水稻防御将被确定。这些信息，沿着其他数据，将用于功能基因组学研究，特别是微阵列分析和基因敲除，从病原体和宿主的角度揭示宿主-病原体识别的许多早期事件。因此，将为植物，细胞和病原体生物学研究社区的分配和长期使用创造重要的新资源。为了方便获取资源和研究数据，将开发一个新的数据库（MGOS），作为植物和病原体研究界之间的桥梁。将在研究的基础上，为高中生设计和开发一个互动教育部分“基因组学和粮食安全”。该项目的一个主要组成部分是促进来自代表性不足群体的本科生的研究经验，包括成立一个基因组学俱乐部，该俱乐部将整合北卡罗来纳州州立大学的其他植物基因组项目。 50，000个突变体及其相应的表型信息、数码照片和基因组DNA 2. 鉴定和克隆影响真菌渗透和发病机制的突变基因3。 腐殖酸分泌蛋白，植物反应，及其功能4。 微阵列和转录谱实验的结果，在水稻宿主和真菌病原体的基因调控。 来自感染水稻组织的SAGE文库6. 35，000 ESTs来自不同的水稻组织感染和非感染7. 公共数据库（MGOS）存储本项目过程中生成的所有数据。 教学模块“基因组学和食品安全”为高中教师和学生联系信息的可接受性：1. 项目负责人，托马斯米切尔，北卡罗来纳州州立大学，[e-mail]，2. 项目主任，拉尔夫·迪恩，北卡罗来纳州州立大学，[e-mail]，3. 生物信息学，Cari Soderlund，亚利桑那大学，[e-mail]4。 教育和推广，布伦达Wojnowski，北卡罗来纳州州立大学，[e-mail]互联网（网络或电子邮件）的项目信息：1。 http://www.fungalgenomics.ncsu.edu2. http://www.riceblast.org3. [e-mail]4. [e-mail]