Development and Application of On-Line cITP-NMR for Analysis of Mass-Limited Samples

用于分析质量限制样品的在线 cITP-NMR 的开发和应用

• 批准号: 0616811
• 负责人: Cynthia Larive
• 金额: $ 44.96万
• 依托单位: University of California-Riverside
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0616811&HistoricalAwards=false
• 关键词: Development; Application; Line; cITP; NMR

Professor Cynthia K. Larive of the University of California, Riverside is supported by the Analytical and Surface Chemistry Program in the Division of Chemistry to work on the development of capillary isotachophoresis- Nuclear magnetic resonance (cITP-NMR) methodology, and to apply this concentration, separation, and dual detection analytical tool for the analysis and structural determination of mass limited samples and heparin oligosaccarides. Analyte bands will be detected by conductivity, and the bands will be focused in the NMR microcoil probe to permit signal averaging and 2D NMR experiments. Detection and structure determination of low-level impurities is a critical component of drug development and discovery, and the use of cITP-NMR will appreciably reduce the mass of isolated oligosaccharides and other chemicals and biochemicals required for NMR analysis. This research will advance the instrumentation for on-line cITP-NMR and will impact analytical chemistry, biochemistry and the pharmaceutical industry. In addition, this project will educate students, including undergraduates and members of underrepresented groups in state of the art analytical instrumentation and methodology.

加州大学河滨分校的 Cynthia K. Larive 教授在化学系分析和表面化学项目的支持下，致力于开发毛细管等速电泳-核磁共振 (cITP-NMR) 方法，并将这种浓缩、分离和双重检测分析工具应用于质量限制样品和肝素寡糖的分析和结构测定。 分析物条带将通过电导率进行检测，条带将聚焦在 NMR 微线圈探针中，以进行信号平均和 2D NMR 实验。低含量杂质的检测和结构测定是药物开发和发现的关键组成部分，使用 cITP-NMR 将显着减少 NMR 分析所需的分离寡糖和其他化学品和生物化学品的质量。这项研究将推进在线 cITP-NMR 仪器的发展，并将影响分析化学、生物化学和制药行业。 此外，该项目还将向学生（包括本科生和弱势群体成员）提供最先进的分析仪器和方法方面的教育。