Advancing cITP-NMR and Reverse-phase Ion-Pairing Separations of Anionic Oligosaccharides

推进阴离子寡糖的 cITP-NMR 和反相离子对分离

• 批准号: 0848976
• 负责人: Cynthia Larive
• 金额: $ 42万
• 依托单位: University of California-Riverside
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0848976&HistoricalAwards=false
• 关键词: Advancing; cITP; NMR; Reverse; phase

Professor Cynthia K. Larive of the University of California, Riverside is supported by the Analytical and Surface Chemistry Program in the Division of Chemistry to work on the development of capillary isotachophoresis-nuclear magnetic resonance (cITP-NMR) methodology for the analysis and structure determination of oligosaccharides from important biopolymers like heparin and chondroitin sulfate. This work also aims to advance methods for the separation of these molecules by reverse-phase ion-pairing chromatography and includes magic angle spinning NMR experiments as an aid to understanding the nature of the intermolecular interactions among the analyte, the ion pairing reagent, and the chromatographic stationary phase.This research offers potential applications in biochemistry and the pharmaceutical industry, which rely heavily on separation methods for compound purification and characterization. In addition, the work offers students (including undergraduates and members of underrepresented groups) opportunities for experience with state-of-the-art analytical instrumentation and methodology. Research advances will be incorporated in the classroom, bringing the excitement of research to students at an early stage of their education. Educational aspects of this work will be disseminated through the Analytical Sciences Digital Library, http://www.asdlib.org, a free portal of peer-reviewed and annotated electronic resources for teachers, learners and practitioners of analytical science.

Cynthia K.加州大学滨江分校的Larive得到化学系分析和表面化学项目的支持，致力于开发毛细管等速-核磁共振（cITP-NMR）方法，用于分析和确定肝素和硫酸软骨素等重要生物聚合物中寡糖的结构。本工作还旨在通过反相离子对色谱法改进这些分子的分离方法，并包括魔角旋转NMR实验，以帮助理解分析物、离子对试剂和色谱固定相之间分子间相互作用的性质。其严重依赖于用于化合物纯化和表征的分离方法。 此外，这项工作还为学生（包括本科生和代表性不足的群体成员）提供了使用最先进的分析仪器和方法的机会。 研究进展将被纳入课堂，在他们的教育的早期阶段为学生带来研究的兴奋。 这项工作的教育方面将通过分析科学数字图书馆http://www.asdlib.org传播，这是一个免费的门户网站，为教师，学习者和分析科学从业者提供同行评审和注释的电子资源。