An Integrative Approach to Elucidate RNA Replication and Systemic Trafficking
阐明 RNA 复制和系统性贩运的综合方法
基本信息
- 批准号:0620143
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2006
- 资助国家:美国
- 起止时间:2006-08-01 至 2009-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
How basic processes in individual cells are integrated at the organismal level to control development and behavior is a major question in current biology. RNA replication and trafficking are emerging as a new paradigm to develop an understanding of the biological principles that integrate systemic gene regulation, viral infection, cellular antiviral responses, and basic mechanisms of cell-to-cell communication. This project addresses the fundamental and yet poorly understood question of how an RNA is replicated by the nuclear machinery and is further selected for transport into neighboring cells and distant organs in a plant, by using Potato spindle tuber viroid (PSTVd) as a highly tractable model. This small (359 nucleotides) infectious RNA has the capacity to replicate in the nucleus and traffic between cells and organs to establish infection. Because it does not have protein-coding capacity and helper viruses, this RNA must have evolved distinct structural motifs to exploit preexisting cellular machineries to accomplish replication and trafficking. Using a combination of biochemical, cellular, genetic, molecular and virological approaches, this project will (1) investigate the PSTVd RNA motifs that play specific roles in replication, (2) isolate and characterize the role of a cellular factor, RNA ligase, in replication, and (3) dissect the RNA motifs that mediate trafficking between specific cells. Results from the project are expected to provide new insights about how an infectious RNA utilizes the host cellular machinery to establish infection, and to help develop a conceptual framework for further studies on the mechanisms and functions of RNA replication and trafficking underlying many biological processes. This project will also train undergraduate/graduate students and postdoctoral fellows in developing cutting-edge multidisciplinary approaches to address fundamental biological problems in great depth and breadth.
个体细胞中的基本过程如何在生物体水平上整合以控制发育和行为是当前生物学中的一个主要问题。RNA复制和运输正在成为一种新的范式,以发展对整合系统性基因调控、病毒感染、细胞抗病毒反应和细胞间通讯基本机制的生物学原理的理解。该项目解决了一个基本的但知之甚少的问题,即RNA是如何通过核机制复制的,并通过使用马铃薯纺锤体块茎类病毒(PSTVd)作为一个高度易处理的模型,进一步选择运输到植物的邻近细胞和远端器官。这种小的(359个核苷酸)感染性RNA具有在细胞核中复制并在细胞和器官之间运输以建立感染的能力。因为它没有蛋白质编码能力和辅助病毒,这种RNA必须进化出独特的结构基序,以利用预先存在的细胞机制来完成复制和运输。利用生物化学、细胞学、遗传学、分子学和病毒学方法的组合,该项目将(1)研究在复制中发挥特定作用的PSTVd RNA基序,(2)分离和表征细胞因子RNA连接酶在复制中的作用,以及(3)解剖介导特定细胞之间运输的RNA基序。该项目的结果有望为感染性RNA如何利用宿主细胞机制建立感染提供新的见解,并有助于为进一步研究许多生物过程中RNA复制和贩运的机制和功能建立概念框架。该项目还将培训本科生/研究生和博士后研究员,以开发尖端的多学科方法,深入和广泛地解决基本生物学问题。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Biao Ding其他文献
Primary-dendrite-pattern regulation by secondary branch during the directional solidification of the single crystal superalloy
单晶高温合金定向凝固过程中二次枝晶臂对一次枝晶间距的调控
- DOI:
10.1016/j.matchar.2023.113208 - 发表时间:
2023-10-01 - 期刊:
- 影响因子:5.500
- 作者:
Xiaotan Yuan;Xingjin Hu;Congjiang Zhang;Hongbin Yu;Weili Ren;Biao Ding;Haibiao Lu;Yunbo Zhong;Zuosheng Lei;Hui Wang;Qiuliang Wang;Peter K. Liaw;Xuezhi Qin;Lanzhang Zhou - 通讯作者:
Lanzhang Zhou
Evolution of Solid–Liquid Interface Influenced by Fluid Flow During Transverse Static Magnetic Field-Electric Current-Assisted Directional Solidification
- DOI:
10.1007/s11663-024-03321-w - 发表时间:
2024-10-23 - 期刊:
- 影响因子:3.100
- 作者:
Ganpei Tang;Boyi Luo;Zhe Sun;Meilong Feng;Wenhao Lin;Zhibin Xia;Zhongze Lin;Tianxiang Zheng;Bangfei Zhou;Peijian Shi;Qiang Li;Chunmei Liu;Zhe Shen;Biao Ding;Yunbo Zhong - 通讯作者:
Yunbo Zhong
Revealing the influential mechanism of strain ranges on cyclic-life saturation during creep-fatigue in Nickel-based superalloy DZ445
揭示镍基高温合金DZ445蠕变疲劳过程中应变范围对循环寿命饱和度的影响机制
- DOI:
10.1016/j.ijplas.2022.103320 - 发表时间:
2022-08 - 期刊:
- 影响因子:9.8
- 作者:
Biao Ding;Weili Ren;Yunbo Zhong;Xiaotan Yuan;Tianxiang Zheng;Zhe Shen;Yifeng Guo;Qiang Li;Chunmei Liu;Jianchao Peng;Josip Brnic;Yanfei Gao;Peter K. Liaw - 通讯作者:
Peter K. Liaw
Understanding the formation and evolution of low-angle grain boundaries in Nickel-Based single crystal superalloys
理解镍基单晶高温合金中低角度晶界的形成与演化
- DOI:
10.1016/j.matchar.2024.113733 - 发表时间:
2024-03-01 - 期刊:
- 影响因子:5.500
- 作者:
Xiaotan Yuan;Congjiang Zhang;Weili Ren;Biao Ding;Haibiao Lu;Yunbo Zhong;Zuosheng Lei;Tianxiang Zheng;Qiang Li;Hui Wang;Qiuliang Wang;Peter K. Liaw;Xuezhi Qin;Lanzhang Zhou - 通讯作者:
Lanzhang Zhou
Primary-dendrite-pattern regulation by secondary branch during the directional solidification of the single crystal superalloy
单晶高温合金定向凝固过程中二次枝晶臂对一次枝晶间距的调控
- DOI:
10.1016/j.matchar.2023.113208 - 发表时间:
2023-10-01 - 期刊:
- 影响因子:5.500
- 作者:
Xiaotan Yuan;Xingjin Hu;Congjiang Zhang;Hongbin Yu;Weili Ren;Biao Ding;Haibiao Lu;Yunbo Zhong;Zuosheng Lei;Hui Wang;Qiuliang Wang;Peter K. Liaw;Xuezhi Qin;Lanzhang Zhou - 通讯作者:
Lanzhang Zhou
Biao Ding的其他文献
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{{ truncateString('Biao Ding', 18)}}的其他基金
Viroid models to study evolution of RNA trafficking motifs for host adaptation
类病毒模型研究宿主适应的RNA运输基序的进化
- 批准号:
1051655 - 财政年份:2011
- 资助金额:
-- - 项目类别:
Continuing Grant
Conference Plant Vascular Biology 2010 held July 24-28, 2010 at Ohio State University Columbus, OH.
2010 年植物血管生物学会议于 2010 年 7 月 24 日至 28 日在俄亥俄州哥伦布市俄亥俄州立大学举行。
- 批准号:
0956232 - 财政年份:2010
- 资助金额:
-- - 项目类别:
Standard Grant
An Integrative Approach to Elucidate Systemic RNA Trafficking
阐明系统性 RNA 贩运的综合方法
- 批准号:
0840906 - 财政年份:2009
- 资助金额:
-- - 项目类别:
Continuing Grant
Novel biogenesis and function of small RNAs derived from a plant pathogen
植物病原体小RNA的新生物合成和功能
- 批准号:
0515745 - 财政年份:2005
- 资助金额:
-- - 项目类别:
Standard Grant
Mechanisms of phloem-mediated RNA traffic
韧皮部介导的 RNA 运输机制
- 批准号:
0238412 - 财政年份:2003
- 资助金额:
-- - 项目类别:
Continuing grant
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