SGER: Nanoparticle Core Virus-like Particles for Intracellular Probing

SGER：用于细胞内探测的纳米核心病毒样颗粒

• 批准号: 0631982
• 负责人: Bogdan Dragnea
• 金额: --
• 依托单位: Indiana University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2007-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0631982&HistoricalAwards=false
• 关键词: SGER; Nanoparticle; Core; Virus; like

Dragnea0631982ABSTRACTThe proposed research will elucidate the general principles underlying the synthesis of virus-likeparticles (VLP), which are novel bio-inspired hybrid systems composed of an icosahedral virus protein coat encapsulating a nanoparticle core. Such VLPs have potential practical utility as biomedical imaging and sensing tools, as novel functional materials, and as experimental models for molecular self-assembly of quasi-spherical molecular cages. VLP-based approaches to biological and biophysical problems go beyond existing technologies since: (1) As nonintrusive optical or magnetic intracellular probes, they push the spatial resolution limits by at least one order of magnitude. (2) As biological vectors, VLPs possess a regular surface structure, which allows the attachment of ligands at well-defined positions characterized by equivalent environments. (3) Principles of viral-entry can be exploited for non-intrusive introduction of nanoparticles inside cells. (4) As biophysical models for molecular cage self-assembly, VLPs benefit of tunable interactions between the inorganic core and the capsid. This feature is currently absent from models for virus assembly.

Dragnea0631982摘要该研究将阐明病毒样颗粒（VLP）合成的一般原理，VLP是由二十面体病毒蛋白外壳包裹纳米颗粒核心组成的新型生物启发的混合系统。这种VLP具有潜在的实际效用，作为生物医学成像和传感工具，作为新型功能材料，并作为准球形分子笼的分子自组装的实验模型。基于VLP的生物学和生物物理学问题的方法超越了现有技术，因为：（1）作为非侵入性的光学或磁性细胞内探针，它们将空间分辨率限制推高了至少一个数量级。(2)作为生物载体，VLP具有规则的表面结构，其允许在由等效环境表征的明确定义的位置处连接配体。(3)可以利用病毒进入的原理将纳米颗粒非侵入性地引入细胞内。(4)作为分子笼自组装的生物物理模型，VLP受益于无机核心和衣壳之间的可调相互作用。目前病毒组装模型中没有此功能。