Biophotonics: In-vitro single-virus near-field spectroscopy

生物光子学：体外单病毒近场光谱学

• 批准号: 0322767
• 负责人: Bogdan Dragnea
• 金额: $ 54.06万
• 依托单位: Indiana University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-08-15 至 2007-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0322767&HistoricalAwards=false
• 关键词: Biophotonics; vitro; single; virus; near

0322767DragneaThe approach proposed here is to avoid averaging over different sub-populations by studying single viruses and to obtain time-resolved structural information in a controlled physiological environment. To this end, because of the extremely small amount of sample in a single virus, utilization of spectroscopic enhancement techniques is required. Functionalized gold nanoparticles introduced into the viral cavity, will play the role of spectroscopic enhancers. In-vitro expansion of the single capsid will be induced by pH variations and monitored by two spectroscopic techniques: surface plasmon resonance spectroscopy and chemically-specific surface enhanced Raman scattering spectroscopy. The goal is to obtain a quantitative measure of the capsid expansion and the number of intermediate transitions.

0322767 Dragnea本文提出的方法是通过研究单个病毒来避免对不同亚群进行平均，并在受控的生理环境中获得时间分辨的结构信息。 为此，由于单个病毒中的样品量非常小，因此需要利用光谱增强技术。 将功能化的金纳米粒子引入到病毒腔中，将起到光谱增强剂的作用。 单个衣壳的体外扩增将通过pH变化诱导并通过两种光谱技术监测：表面等离子体共振光谱和化学特异性表面增强拉曼散射光谱。 目标是获得衣壳扩增和中间转变的数量的定量测量。