SGER: Strategies for Increasing Stability of Self-Assembling DNA Nanostructures

SGER：提高自组装DNA纳米结构稳定性的策略摘要：Thomas H. LaBeanDNA纳米结构自组装是一种“自下而上”的方法，用于制造所需的模式和结构，其特征尺寸比传统刻印技术可实现的要小。本文提出的探索性研究旨在通过在DNA构建块内部和之间结合isoG/isoC碱基对，促进相邻寡核苷酸之间的连接以及探索链间化学交联来提高DNA纳米结构的稳定性。该项目的长期目标是开发稳定DNA和生物分子纳米结构的方法，以提高产量，改善半衰期，并使结构可用的物理和化学条件多样化。此外，已经注意到，在组装成晶格之前，将纳米材料（例如金属纳米晶体或大蛋白质）附着在组成dna链上可能会导致组装困难并减小所产生的超结构的尺寸。稳定晶格可能有助于克服这些问题。拟研究任务概述：1)。开发DNA自组装材料和技术，以形成更大的可寻址阵列和晶格。2)。结合稳定策略，包括isog /isoC和连接到DNA瓦片晶格设计。进行纳米级结构表征。3）.研究DNA构建块内通过化学桥连接的链间交联。4)。测试稳定的纳米结构作为金属沉积模板和固定化导电、半导体和介电纳米材料的支架。拟议的项目通过利用生物系统和分子工程等不同领域的进步和见解，提高计算机科学和工程的基本能力，实现了CCF部门和新兴模型和技术计划的目标。提出的研究创新使计算和通信硬件制造的方法从根本上不同。所提议的活动的智力价值很高，因为该项目将DNA自组装的前沿研究与新的合成碱基对选择和化学交联程序结合在一起，而且pi实验室目前具有独特的资格和准备执行所提议的研究。有很高的可能性，研究任务将完成描述和非常高的科学和技术回报，当他们成功。提议的工作的更广泛的影响包括稳定的DNA纳米结构的可能性，不仅应用于计算，而且应用于生物医学用途，以及为下一代生物灵感设计，分子工程，自组装和纳米结构模板化学的创造性专家建立研究培训机会的确定性。

SGER: Strategies for Increasing Stability of Self-Assembling DNA NanostructuresPI: Thomas H. LaBeanDNA nanostructure self-assembly is being developed as a "bottom-up" approach for thefabrication of desired patterns and structures with feature sizes smaller than achievable with conventionallithography techniques. The exploratory research proposed here aims to increase the stability of DNAnanostructures by incorporating isoG/isoC base pairs within and between DNA building blocks, byfacilitating ligation between abutting oligonucleotides, and by exploring interstrand chemical crosslinking.The long-term goal of the proposed project is to develop methods for stabilizing DNA andbiomolecular nanostructures to increase production yields, improve the halflife, and diversify the physicaland chemical conditions under which the structures are useable. In addition, it has been noted, thatattachment of nanomaterials, for example metallic nanocrystals or large proteins, to the component DNAstrands prior to assembly into lattices can result in assembly difficulties and decreased size of theresulting superstructures. Stabilized lattices may help to overcome these issues.Summary of Proposed Research Tasks: 1). Develop DNA self-assembly materials and techniquesfor formation of larger addressable arrays and lattices. 2). Incorporate stabilizing strategies, includingisoG/isoC and ligation into DNA tile lattice designs. Perform nanoscale structural characterization. 3).Investigate interstrand cross-linking via chemical bridges within DNA building blocks. 4). Test thestabilized nanostructures as templates for metal deposition and scaffolds for immobilization ofconducting, semi-conducting, and dielectric nanomaterials.The proposed project fulfills the goals of the CCF Division and of the Emerging Models andTechnologies Program by advancing fundamental capabilities in computer science and engineering usingadvances and insights from areas as diverse as biological systems and molecular engineering. Theproposed research innovations enable fundamentally different ways of approaching the fabrication ofcomputing and communications hardware. The intellectual merit of the proposed activities is highbecause the project brings together cutting-edge research in DNA self-assembly with new syntheticbasepair options and chemical cross-linking procedures, and from the fact that the PIs lab is uniquelyqualified and poised to execute the proposed study at this time. There is a high likelihood that the researchtasks will be completed as described and very high scientific and technological payoffs when theysucceed. Broader impacts of the proposed work include the possibility of stabile DNA nanostructures forapplication not only to computing but also to biomedical uses, as well as the certainty of establishingresearch training opportunities for the next generation of creative experts in bioinspired design, molecularengineering, self-assembly, and nanostructure templated chemistry.