MRI: Instrument Acquisition to Enable Time-Resolved Single-Molecule Fluorescence Measurements at Wheaton College

MRI：惠顿学院利用仪器采集实现时间分辨单分子荧光测量

• 批准号: 0722688
• 负责人: Daniel Burden
• 金额: $ 38.58万
• 依托单位: Wheaton College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-15 至 2010-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0722688&HistoricalAwards=false
• 关键词: MRI; Instrument; Acquisition; Enable; Time

The Chemistry Department at the Wheaton College will acquire a cluster of instruments to make their single-molecule microscopy and laser laboratory at Wheaton College capable of picosecond to nanosecond time-resolved fluorescence measurements. A high-power, picosecond-pulsed laser will be configured for multi-user access with data acquisition hardware that includes: (1) a time correlated single photon counting (TCSPC) PC card; (2) a stage, interface, and software for fluorescence lifetime imaging (FLIM); (3) a fluorescence lifetime spectrometer; (4) electronics and software for time-gated optical and electrical detection of single ion channels in large lipid bilayer membranes. The specific research contributions facilitated by this instrumentation request include: (1) the development of a novel time-gated detection scheme that will expand the detection area for single molecules in the far-field by ~100-fold over the existing state-of-the-art technique; (2) a comparison of the dynamics and structure of alpha hemolysin and Anthrax toxins in lipid membranes; (3) a study of electron transfer and solvation dynamics in polyelectrolyte multilayer (PEM) thin films; (4) the development of new time-resolved fluorescence assays for structure/function studies of cGMP-dependent protein kinase.Fluorescence instrumentation provides an excellent means for fostering interdisciplinary scientific investigation because fluorescence applications are replete throughout chemistry, biology, and physics. Fluorescence measurements record the emission of radiation after being probed with a laser or light source. They are inherently sensitive, enabling trace amounts of material to be probed. Once established, the techniques are relatively simple and robust, which facilitates multi-user accessibility. The equipment will be used by undergraduates in research and laboratory instruction.

惠顿学院化学系将获得一组仪器，使他们在惠顿学院的单分子显微镜和激光实验室能够进行皮秒到纳秒的时间分辨荧光测量。高功率皮秒脉冲激光器将配置用于多用户访问的数据采集硬件，包括：(1)时间相关单光子计数（TCSPC） PC卡；(2)荧光寿命成像（FLIM）的平台、界面和软件；(3)荧光寿命光谱仪；(4)用于大脂质双层膜中单离子通道的时间门控光学和电检测的电子和软件。该仪器的具体研究贡献包括：(1)开发了一种新的时间门控检测方案，该方案将远场单分子的检测范围扩大到现有最先进技术的100倍；(2)脂质膜α溶血素和炭疽毒素的动态和结构比较；(3)聚电解质多层（PEM）薄膜中的电子转移和溶剂化动力学研究；(4)开发新的时间分辨荧光法用于cgmp依赖性蛋白激酶的结构/功能研究。荧光仪器为促进跨学科的科学研究提供了一种极好的手段，因为荧光的应用在化学、生物学和物理学中都很广泛。荧光测量记录了用激光或光源探测后的辐射发射。它们天生敏感，可以探测到微量的物质。一旦建立起来，这些技术就相对简单和健壮，有助于多用户访问。该设备将用于本科生的研究和实验室教学。