An integrated approach, using biofabrication and chemical synthesis, to study cell signaling

1264509 Sintim, Herman O.该奖项支持马里兰大学帕克分校的Herman O. Sintim、William Bentley和Gary Rubloff教授的研究。该团队将开发多模式和多功能微流体系统，通过将刺激响应生物制造与设备施加的复杂梯度生成细菌信号分子相结合，用于研究细胞信号。该装置将用于系统地研究细菌细胞对群体感应分子和环境信号的反应。此外，该设备将用于识别抑制细菌趋化性的新分子。由于细菌细胞信号在细菌生理学中的核心作用，人们对理解或揭示控制细菌对信号分子反应的各种因素非常感兴趣。能够帮助筛选抑制细菌交流的分子的变革性技术将在医学、农业和工业中有潜在的应用。这个项目更广泛的影响包括开发一个新的技术平台来研究细菌如何相互作用和环境。这个项目是高度跨学科的，涉及化学生物学家、生物工程师和生物系统工程师。因此，参与该项目的学生将接受训练，以使用不同的方法解决科学问题。该奖项由CBET部门的生物技术、生化和生物质工程项目颁发，由分子和细胞生物学部门的系统和合成生物学项目共同资助。

1264509 Sintim, Herman O.This award is supporting the research of Professors Herman O. Sintim, William Bentley and Gary Rubloff of the University of Maryland at College Park. The team will develop multimodal and multifunctional microfluidic systems for studying cell signaling by integrating stimuli-responsive biofabrication with device-imposed, complex gradient generation of bacterial signaling molecules. This device will then be used to systematically investigate the response of bacterial cells to quorum sensing molecules and environmental cues. Additionally, the device will be used to identify new molecules that inhibit bacterial chemotaxis.Due to the central role that bacterial cell signaling plays in bacterial physiology, there is a high interest in understanding or unraveling the various factors that control bacterial response to signaling molecules. Transformative technologies that can aid the screening of molecules that inhibit bacterial communication would have potential applications in medicine, agriculture and industry. The broader impact of this project includes development of a new technological platform to study how bacteria interact with each other and the environment. This project is highly multidisciplinary, involving chemical biologists, bioengineers and biosystems engineers. Therefore, the students who will be involved with the project will be trained to solve scientific problems using diverse approaches.This award by the Biotechnology, Biochemical, and Biomass Engineering Program of the CBET Division is co-funded by the Systems and Synthetic Biology Program of the Division of Molecular and Cellular Biology.