An integrated approach, using biofabrication and chemical synthesis, to study cell signaling

1264509 Sintim，赫尔曼O.该奖项是支持教授赫尔曼O.马里兰州大学帕克分校的辛蒂姆、威廉·本特利和加里·鲁布洛夫说。该团队将开发多模式和多功能微流体系统，用于通过将刺激响应生物纤维素与细菌信号分子的设备施加的复杂梯度生成相结合来研究细胞信号传导。然后，该装置将用于系统地研究细菌细胞对群体感应分子和环境线索的反应。此外，该装置将用于识别抑制细菌趋化性的新分子。由于细菌细胞信号在细菌生理学中起着核心作用，因此人们对理解或解开控制细菌对信号分子反应的各种因素非常感兴趣。能够帮助筛选抑制细菌通讯的分子的变革性技术将在医学，农业和工业中具有潜在的应用。该项目的更广泛影响包括开发一个新的技术平台，以研究细菌如何相互作用和环境。该项目是高度多学科的，涉及化学生物学家，生物工程师和生物系统工程师。因此，将参与该项目的学生将接受培训，以使用不同的方法来解决科学问题。该奖项由CBET部门的生物技术，生物化学和生物质工程项目共同资助，由分子和细胞生物学部门的系统和合成生物学项目共同资助。

1264509 Sintim, Herman O.This award is supporting the research of Professors Herman O. Sintim, William Bentley and Gary Rubloff of the University of Maryland at College Park. The team will develop multimodal and multifunctional microfluidic systems for studying cell signaling by integrating stimuli-responsive biofabrication with device-imposed, complex gradient generation of bacterial signaling molecules. This device will then be used to systematically investigate the response of bacterial cells to quorum sensing molecules and environmental cues. Additionally, the device will be used to identify new molecules that inhibit bacterial chemotaxis.Due to the central role that bacterial cell signaling plays in bacterial physiology, there is a high interest in understanding or unraveling the various factors that control bacterial response to signaling molecules. Transformative technologies that can aid the screening of molecules that inhibit bacterial communication would have potential applications in medicine, agriculture and industry. The broader impact of this project includes development of a new technological platform to study how bacteria interact with each other and the environment. This project is highly multidisciplinary, involving chemical biologists, bioengineers and biosystems engineers. Therefore, the students who will be involved with the project will be trained to solve scientific problems using diverse approaches.This award by the Biotechnology, Biochemical, and Biomass Engineering Program of the CBET Division is co-funded by the Systems and Synthetic Biology Program of the Division of Molecular and Cellular Biology.