US-Tunisia Planning Visit: The Genomics of Salinity Adaptation in the Medicago Truncatula-Sinorhizobium Symbiosis

美国-突尼斯计划访问：蒺藜苜蓿-中华根瘤菌共生中盐度适应的基因组学

• 批准号: 0751073
• 负责人: Sergey Nuzhdin
• 金额: $ 1.96万
• 依托单位: University of Southern California
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0751073&HistoricalAwards=false
• 关键词: US; Tunisia; Planning; Visit; Genomics

0751073 NuzhdinThis proposal is to support planning visits by the two PIs, Dr. Sergey Nuzhdin, Department of Molecular & Computational Biology, University of Southern California, Los Angeles and Dr. Sharon Strauss, Section of Evolution and Ecology, University of California at Davis, California to Tunisia, to establish research collaboration between them and Dr. Mohamed Elarabi Aouani, Center of Biotechnology, Technopole de Bordj Cedra (CBBC) in Hammam Lif, Tunisia. The PIs collaboration will also involve a number of French scientists working with Dr. Aouani. They plan to discuss the Genomics of Salinity Adaptation in the Medicago truncatula-Sinorhizobium symbiosis. They will participate in extensive discussions and tour the Tunisian lab, greenhouse facilities, and field sites. Dr. Aouani's team will contribute biological material, perform genetic characterization and greenhouse trials, and participate in field experiments during the course of the proposed project.Intellectual merit: Although Darwin emphasized the importance of ecological interactions for evolutionary diversification, we have only recently acquired the molecular tools that allow us to understand how adaptive variation evolves in response to environmental challenges and ecological interactions. The project will focus on an ecographically diverse Tunisian collection of Medicago truncatula (Mtr) and their nitrogen-fixing symbionts Sinorhizobium meliloti (Sm). The primary research goal is to identify networks of genes in the Mtr and Sm genomes that are involved in adaptation and coadaptation in response to high-salinity conditions. Genotypes from six focal populations, three from highly saline sites, will be tested under high vs. low salinity and in factorial combinations with rhizobial strains from high and low salinity sites, to document the roles of Mtr genotype, Sm genotype, and their interaction on performance under contrasting salinity regimes. Concurrent metabolome and transcriptome profiles will identify networks of genes associated with salinity tolerance and fitness tradeoffs between experimental treatments. Broader impacts: Increasing soil salinity is a major agricultural problem that will only becomemore urgent with continued irrigation. A long-term goal of this project is to facilitate thedevelopment of legume crops suited to saline environments, by identifying genetic pathways involved in salinity tolerance, and determining how these pathways influence the relationship of the host plant with its nitrogen-fixing symbiont. Because disparate regions of the world face similar environmental challenges, cross-cultural exchanges of knowledge and training are essential for science-based problem solving. The project will involve support for a U.S. post-doctoral scientist and a U.S. graduate student to participate in the visits.

0751073 Nuzhdin 此提案旨在支持两位 PI（南加州大学洛杉矶分校分子与计算生物学系的 Sergey Nuzhdin 博士和加州大学戴维斯分校进化与生态学系的 Sharon Strauss 博士）计划访问突尼斯，以建立他们与突尼斯中心的 Mohamed Elarabi Aouani 博士之​​间的研究合作 生物技术，突尼斯 Hammam Lif 的 Technopole de Bordj Cedra (CBBC)。 PI 的合作还将涉及一些法国科学家与 Aouani 博士的合作。 他们计划讨论蒺藜苜蓿-中华根瘤菌共生中盐度适应的基因组学。 他们将参加广泛的讨论并参观突尼斯实验室、温室设施和现场。 Aouani 博士的团队将在拟议项目过程中贡献生物材料，进行遗传表征和温室试验，并参与实地实验。智力价值：尽管达尔文强调了生态相互作用对于进化多样化的重要性，但我们最近才获得了分子工具，使我们能够了解适应性变异如何进化以应对环境挑战和生态相互作用。该项目将重点关注突尼斯生态多样化的蒺藜苜蓿 (Mtr) 及其固氮共生体苜蓿中华根瘤菌 (Sm)。主要研究目标是确定 Mtr 和 Sm 基因组中参与响应高盐度条件的适应和共适应的基因网络。来自六个焦点种群（其中三个来自高盐度地点）的基因型将在高盐度和低盐度下进行测试，并与来自高盐度和低盐度地点的根瘤菌菌株进行因子组合，以记录 Mtr 基因型、Sm 基因型的作用以及它们在对比盐度条件下对性能的相互作用。同时的代谢组和转录组谱将识别与盐度耐受性和实验处理之间的适应性权衡相关的基因网络。更广泛的影响：土壤盐分增加是一个主要的农业问题，随着持续灌溉，这一问题只会变得更加紧迫。该项目的长期目标是通过识别与耐盐性相关的遗传途径，并确定这些途径如何影响宿主植物与其固氮共生体的关系，促进适合盐分环境的豆科作物的发展。 由于世界不同地区面临相似的环境挑战，跨文化的知识和培训交流对于以科学为基础的问题解决至关重要。该项目将支持一名美国博士后科学家和一名美国研究生参加访问。