Physical and Chemical Directors of Molecular Asymmetry in Lipid Bilayers

脂质双层分子不对称的物理和化学指导

• 批准号: 0808923
• 负责人: John Conboy
• 金额: $ 46.5万
• 依托单位: University of Utah
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-15 至 2012-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0808923&HistoricalAwards=false
• 关键词: Physical; Chemical; Directors; Molecular; Asymmetry

The Analytical and Surface Chemistry (ASC) program of the Division of Chemistry will support the research program of Prof. John Conboy of the University of Utah. Prof. Conboy and his students will study the origin of phospholipid asymmetry in simple model membrane systems. Compositional asymmetry in the lipid membrane is thought to play a crucial role in membrane structural stability, intercellular signaling, and the organization of membrane-associated components. Prof. Conboy and his students will use sum frequency vibrational spectroscopy (SFVS) to directly measure the trans bilayer movement of phospholipids and asymmetry in a variety of membrane models to determine the rate of exchange of native phospholipids. SFVS will also be used to measure membrane asymmetry, kinetics of transmembrane migration, and thermodynamic analysis of the transition state barrier to migration. Success of the proposed studies will have wide-reaching implications in molecular and cellular biology. The study will provide excellent educational training opportunities for graduate students and postdoctoral trainees in a highly multidisciplinary research area involving the development and application of state of the art no-linear spectroscopic methods in complex biological systems.

化学系的分析和表面化学（ASC）项目将支持犹他大学John Conboy教授的研究项目。康博伊教授和他的学生将研究简单模型膜系统中磷脂不对称的起源。脂质膜的成分不对称被认为在膜结构稳定性、细胞间信号传导和膜相关成分的组织中起着至关重要的作用。Conboy教授和他的学生将使用和频振动光谱（SFVS）直接测量各种膜模型中磷脂的跨双层运动和不对称性，以确定天然磷脂的交换速率。SFVS还将用于测量膜的不对称性、跨膜迁移动力学和迁移过渡态势垒的热力学分析。这些研究的成功将对分子和细胞生物学产生深远的影响。该研究将为研究生和博士后学员提供良好的教育培训机会，涉及复杂生物系统中最先进的非线性光谱方法的开发和应用。