STTR Phase I: Magnetic Nanoparticle Microfluidics for High Efficient Capture, Separation and Concetration of Foodborne Pathogens

STTR 第一阶段：用于高效捕获、分离和浓缩食源性病原体的磁性纳米颗粒微流体

• 批准号: 0810626
• 负责人: Yongqiang Wang
• 金额: --
• 依托单位: Ocean NanoTech, LLC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0810626&HistoricalAwards=false
• 关键词: STTR; Phase; Magnetic; Nanoparticle; Microfluidics

This Small Business Technology Transfer Phase I research demonstrates the feasibility to use the new generation of oxide magnetic nanoparticles for rapid and high efficient separation of foodborne pathogens. Rapid detection of foodborne pathogens is urgently needed to ensure food safety and security, and in developing new detection methods, separation of target pathogens at ultra low concentrations (1 - 100 cfu/mL) from food is very critical. The recently developed nanoparticles (4 ? 50 nm) with high magnetization value and well controlled size and surface chemistry show great potential for their use in bioseparation. Therefore, the objective of this project is to apply new oxide magnetic nanoparticles to a portable, inexpensive magnetic separator for rapid and high efficient separation of foodborne pathogens. In Phase I of this project, E. coli O157:H7 will be used as the target bacterium to test magnetic nanoparticles with different sizes and structures, to evaluate antibody-nanoparticle conjugates, different buffer solutions and protocols. The broader impacts of this research are to improve the safety and security of the nation?s food supply and biological systems. The proposed new technology will greatly enhance the rapid detection of pathogens in food products, and thus to reduce foodborne illnesses. Also, the outcome of this proposed research should provide direct economic benefits to the food industry in terms of reduced product recalls and international trade barrier due to microbial contamination.

这项小企业技术转让第一阶段研究证明了使用新一代氧化物磁性纳米颗粒快速高效分离食源性病原体的可行性。食源性致病菌的快速检测是保障食品安全的迫切需要，而在开发新的检测方法时，从食品中分离出超低浓度（1 - 100 cfu/mL）的目标致病菌是非常关键的。最近开发的纳米粒子（4？50 nm），具有高磁化强度和良好的尺寸控制和表面化学性质，在生物分离中具有很大的应用潜力。因此，本研究的目的是将新型氧化物磁性纳米粒子应用于便携式、廉价的磁性分离器中，以快速、高效地分离食源性致病菌。 在本项目的第一阶段，E. coli O 157：H7作为靶菌，测试不同大小和结构的磁性纳米颗粒，评价抗体-纳米颗粒偶联物、不同缓冲溶液和方案。这项研究的更广泛的影响是改善国家的安全和保障？的食物供应和生物系统。拟议的新技术将大大提高食品中病原体的快速检测，从而减少食源性疾病。此外，这项拟议研究的结果应提供直接的经济利益，食品工业方面减少产品召回和国际贸易壁垒，由于微生物污染。