Analysis of the Function of NPH3 and the Role of CUL3-based Ubiquitylation in Phototropic Signaling

NPH3 的功能分析以及基于 CUL3 的泛素化在向光性信号传导中的作用

• 批准号: 0817737
• 负责人: Emmanuel Liscum
• 金额: $ 48万
• 依托单位: University of Missouri-Columbia
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0817737&HistoricalAwards=false
• 关键词: Analysis; Function; NPH3; Role; CUL3

Emmanuel Liscum IOS-0817737Analysis of the function of NPH3 and the role of CUL3-based ubiquitylation in phototropic signalingPlants, unlike animals, are unable to run away from adversity (e.g., herbivores and drought) or toward their next meal (e.g., sunlight and water). Instead plants have evolved strategies that involve organ bending in order to respond to changes in their environment. Dramatic, rapid, and reversible changes in morphology can result from differential growth - unequal cellular elongation in one position of an organ relative to an opposing position. One such response is phototropism, or organ bending in response to directional blue light (BL). Based on preliminary results and recent paradigms developed in fungal and animal systems the investigators hypothesize that NPH3, a protein required for normal phototropism that interacts with the primary phototropic receptor phototropin 1 (phot1), functions as a core component of a CULLIN3-based E3 ubiquitin ligase. They further hypothesize that ubiquitination of phot1 by this complex leads to relocalization of the receptor from the plasma membrane to endomembranes in response to BL, and that this is necessary for the furthered progression of phototropic signals. This project is aimed at addressing this hypothesis by: 1) characterizing phot1 ubiquitylation in response to BL, as well as the role of NPH3 and a CUL3-based E3-ubiquitin ligase in this response; and 2) characterize the influence of ubiquitylation on phot1 localization and phototropic function. Results from these studies will not only provide important insights into the mechanisms by which phototropic signaling occurs, but will also contribute to the blossoming field of CULLIN3-based E3 ligase study, which to this point has been largely dominated by fungal and animal models. This project will provide theoretical and practical training for one technician (female), at least one PhD student (female), one undergraduate student, and two high school students. Genetic resources from the project will be utilized in the PREP (Partnership for Research and Education in Plants) program that the PI participates in to provide hands-on research experience to high school students in Columbia and Jefferson City, MO. Insights into the regulation of plant growth and cellular signaling resulting from these studies, which will published in publicly-accessible and high-profile scientific journals, will hopefully be integrated into current crop improvement programs aimed at increasing economic and ecological aspects of US agriculture.

Emmanuel Liscum IOS-0817737分析 NPH3 的功能以及基于 CUL3 的泛素化在向光性信号传导中的作用植物与动物不同，无法逃离逆境（例如食草动物和干旱）或奔向下一顿饭（例如阳光和水）。 相反，植物进化出了涉及器官弯曲的策略，以应对环境的变化。 形态学上显着、快速和可逆的变化可能是由生长差异引起的——器官的一个位置相对于另一位置的细胞伸长不相等。 其中一种反应是向光性，或响应定向蓝光 (BL) 的器官弯曲。 根据初步结果和最近在真菌和动物系统中开发的范例，研究人员假设 NPH3（一种正常向光性所需的蛋白质，与主要向光性受体向光性受体 趋光性蛋白 1 (phot1) 相互作用）作为基于 CULLIN3 的 E3 泛素连接酶的核心组件发挥作用。 他们进一步假设，该复合物对 phot1 的泛素化导致受体响应 BL 从质膜重新定位到内膜，这对于向光信号的进一步发展是必要的。 该项目旨在通过以下方式解决这一假设：1）表征 BL 响应中的 phot1 泛素化，以及 NPH3 和基于 CUL3 的 E3 泛素连接酶在此响应中的作用； 2) 表征泛素化对 phot1 定位和向光功能的影响。 这些研究的结果不仅将为向光信号发生机制提供重要见解，还将有助于基于 CULLIN3 的 E3 连接酶研究的蓬勃发展，迄今为止，该领域主要由真菌和动物模型主导。 该项目将为一名技术员（女性）、至少一名博士生（女性）、一名本科生和两名高中生提供理论和实践培训。 该项目的遗传资源将用于 PI 参与的 PREP（植物研究和教育合作伙伴关系）计划，为哥伦比亚和密苏里州杰斐逊市的高中生提供实践研究经验。这些研究得出的对植物生长和细胞信号传导调节的见解将发表在公开的高知名度科学期刊上，有望整合到当前旨在提高美国农业经济和生态方面的作物改良计划中。