Analysis of the Regulatory Roles of Protein Phosphorylation and Ubiquitylation in phot1 Endocytosis and Receptor Desensitization

蛋白质磷酸化和泛素化在 phot1 内吞和受体脱敏中的调控作用分析

• 批准号: 1146142
• 负责人: Emmanuel Liscum
• 金额: $ 54.37万
• 依托单位: University of Missouri-Columbia
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-15 至 2016-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1146142&HistoricalAwards=false
• 关键词: Analysis; Regulatory; Roles; Protein; Phosphorylation

The long-term goal of this research is to understand how plants perceive changes in their light environment, transduce that information, and ultimately alter growth and development to elicit appropriate adaptive responses. In this project, the phototropic response, or directional growth of a plant toward or away from light, is being used as a model response to understand the molecular components underlying plant light responses. In particular, physiological, genetic, biochemical, and molecular approaches will be utilized to study the mechanism(s) by which a specific light receptor, phototropin 1, is capable of converting a directional light signal into a physiological response in the plant. Results from these studies will provide important insights not only into the mechanisms of phototropic signaling but receptor-mediated signaling in general. Because surface and intracellular receptors are utilized by all cells (bacterial, fungal, plant, and animal) to sense environmental cues, results from this work has a potential impact on a variety of human endeavors, from agriculture to medicine. Though such impacts are likely long-term, direct and tangible impact of this project on our nation will be realized through training of young scientists (one technician, at least two PhD students, and three undergraduate students). Moreover, the proposed outreach to local K-12 schools will have a clear and immediate impact on science education.

这项研究的长期目标是了解植物如何感知光环境的变化，传递这些信息，并最终改变生长和发育，以引起适当的适应性反应。在这个项目中，植物的趋光性反应，或植物朝向或背离光的定向生长，被用作一个模型反应，以了解植物光反应背后的分子成分。特别是，将利用生理、遗传、生化和分子方法来研究植物中一种特定的光感受器--趋光素1--能够将方向光信号转化为生理反应的机制(S)。这些研究的结果不仅将为光致信号的机制提供重要的见解，而且将在总体上为受体介导的信号的机制提供重要的见解。由于表面和细胞内受体被所有细胞(细菌、真菌、植物和动物)用来感知环境线索，这项工作的结果对从农业到医学的各种人类努力都有潜在的影响。虽然这种影响可能是长期的，但这个项目对我国的直接和切实的影响将通过培训年轻科学家(一名技术人员、至少两名博士生和三名本科生)来实现。此外，拟议扩大到当地K-12学校的活动将对科学教育产生明显和直接的影响。