Lipid bilayer-inorganic nanoparticle interactions: Model systems to examine the role of particle size and surface chemistry on cell membrane stability

脂质双层-无机纳米颗粒相互作用：建立模型系统来检查颗粒尺寸和表面化学对细胞膜稳定性的作用

• 批准号: 0828022
• 负责人: Geoffrey Bothun
• 金额: $ 18.85万
• 依托单位: University of Rhode Island
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0828022&HistoricalAwards=false
• 关键词: Lipid; bilayer; inorganic; nanoparticle; interactions

CBET- 0828022 BothunAs nanomaterial-based commercial technologies and products emerge, it is important that studies be conducted in tandem to gauge their potential health and environmental impacts. Biological membranes define cell structure and function, and provide an initial point of contact for cell/nanomaterial interactions. However, quantifying these interactions and relating them to nanomaterial biocompatibility has proven difficult using in vitro or in vivo studies. This project will examine thermodynamic and transport properties of lipid bilayers, as model membranes, exposed to engineered nanoparticles. The goal is to determine the effect of nanoparticle composition, size, and surface chemistry on biomembrane stability by elucidating specific bilayer/nanoparticle interactions mechanisms. Materials of immediate interest include carbon fullerenes, and native or hydrophobically modified alumina, iron oxide, and silver nanoparticles ranging from 5 to 20 nm. Research will be conducted under four specific aims: (1) Protocols will be developed for examining biomembrane-nanoparticle interactions using model lipid bilayers in the form of vesicles and supported lipid membranes (SLMs, planar). Complimentary information will be obtained from the two geometries. (2) Changes in thermodynamic properties, such as phase behavior and melting cooperativity, associated with biomembrane response to nanoparticles will be characterized. This includes nanoparticles adsorbing within the bilayer (hydrophobic) or at the lipid/water interface (hydrophilic). (3) The ability of adsorbed nanoparticles to alter the lateral diffusion of lipids within the bilayer, which relates to the dynamic behavior of cell membranes, will be examined. (4) Changes in transmembrane permeability due to bilayer stabilization and/or destabilization with adsorbed nanoparticles will be characterized.Nanomaterial toxicity has received considerable attention as of late, and this project addresses a specific aspect related to the cell membrane and nanoparticle bioaccumulation. Understanding such interactions will aid the design of biocompatible nanomaterials. In addition to publications and presentations, they will disseminate their results on their research website and the NIOSH Nanoparticle Information Library website. In addition, the new structures formed will provide interesting hybrid liposomal systems that could potentially be used in the medical and pharmaceutical fields. This project will also serve as an educational tool for high school, undergraduate, and graduate students. The PI participates in a summer high school intern program and has been contacted by the New England LSAMP program to mentor students. These programs target underrepresented students in engineering. Finally, the concepts behind this project and the results obtained will be used as teaching material in a new interdisciplinary graduate-level Bionanotechnology course offered in the spring semester.

CBET- 0828022 Bothun随着基于纳米材料的商业技术和产品的出现，重要的是同时进行研究以评估其潜在的健康和环境影响。 生物膜定义细胞结构和功能，并为细胞/纳米材料相互作用提供初始接触点。 然而，使用体外或体内研究来量化这些相互作用并将其与纳米材料的生物相容性联系起来已被证明是困难的。 该项目将检查暴露于工程纳米粒子的脂质双层（作为模型膜）的热力学和传输特性。目标是通过阐明特定的双层/纳米颗粒相互作用机制来确定纳米颗粒组成、尺寸和表面化学对生物膜稳定性的影响。 人们最感兴趣的材料包括碳富勒烯、天然或疏水改性的氧化铝、氧化铁和 5 至 20 nm 的银纳米颗粒。 研究将根据四个具体目标进行：（1）将开发使用囊泡和支持的脂质膜（SLM，平面）形式的脂质双层模型来检查生物膜-纳米粒子相互作用的方案。 将从这两个几何形状中获得补充信息。 (2) 将表征与生物膜对纳米粒子的响应相关的热力学性质的变化，例如相行为和熔化协同性。 这包括吸附在双层内（疏水性）或脂质/水界面（亲水性）的纳米颗粒。 (3) 将检查吸附的纳米粒子改变双层内脂质横向扩散的能力，这与细胞膜的动态行为有关。 (4) 将表征由于双层稳定和/或吸附纳米颗粒去稳定而导致的跨膜渗透性变化。纳米材料毒性最近受到了相当多的关注，该项目解决了与细胞膜和纳米颗粒生物累积相关的特定方面。 了解这种相互作用将有助于生物相容性纳米材料的设计。 除了出版物和演示之外，他们还将在其研究网站和 NIOSH 纳米颗粒信息图书馆网站上传播他们的研究结果。 此外，形成的新结构将提供有趣的混合脂质体系统，有可能用于医疗和制药领域。 该项目还将作为高中生、本科生和研究生的教育工具。 该 PI 参加了暑期高中实习生计划，并已与新英格兰 LSAMP 计划联系以指导学生。 这些项目针对工程领域代表性不足的学生。 最后，该项目背后的概念和获得的结果将用作春季学期开设的新的跨学科研究生水平生物纳米技术课程的教材。