TT Virus: A Potential Indicator of Human Enteric Viruses in Source and Drinking Waters

TT 病毒：水源和饮用水中人类肠道病毒的潜在指标

• 批准号: 0853459
• 负责人: Sharon Long
• 金额: $ 25.57万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0853459&HistoricalAwards=false
• 关键词: TT; Virus; Potential; Indicator; Human

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).0853459LongIt is widely recognized that bacterial indicators do not co-occur with infectious viruses, nor do they respond in the same manner to environmental or engineered stressors. Thus, a more appropriate indicator for health risks of infectious viruses is needed. The principal objective of this proposal is to study the utility of the Torque Teno (TT) virus as an alternative viral pathogen indicator. TT virus is a small, non-enveloped DNA virus that likely exhibits similar transport characteristics to pathogenic enteric viruses. TT virus is unique among enteric viruses in that it appears to be present ubiquitously in humans; elicits seemingly innocuous infections; does not exhibit seasonal fluctuations or epidemic spikes; is transmitted primarily via the fecal-oral route; and can be assayed using rapid, molecular techniques. This research will be conducted in four phases: (1) to develop a robust TT virus assay, (2) to monitor the density and occurrence of TT virus in sources and source waters, (3) to assess the co-location of TT virus with bacterial indicators, male-specific coliphage, norovirus and adenovirus:, and (4) to compare TT virus data to occurrence and survival data for coliforms, coliphages and enteroviruses. This project will greatly expand the understanding of viral pathogen risk and the utility of TT virus as an indicator system by assessing an as yet untested indicator, and will potentially develop a completely new, reliable, efficient indicator system for viral pathogen risk. The project will include support of students and will offer a multi-disciplinary experience for each. The students will have the opportunity to present results at conferences. , and the investigators are women and have involved underrepresented students in their work previously. The project is expected to contribute to improving public health, and the results will be broadly disseminated.

该奖项是根据2009年美国复苏和再投资法案（公法111 - 5）资助的。0853459长人们普遍认为，细菌指标不与传染性病毒共存，也不以相同的方式对环境或工程应激源作出反应。因此，需要一个更适当的传染性病毒健康风险指标。本提案的主要目的是研究Torque Teno（TT）病毒作为替代病毒病原体指标的效用。TT病毒是一种小的无包膜DNA病毒，可能表现出与致病性肠道病毒相似的转运特征。TT病毒在肠道病毒中是独特的，因为它似乎普遍存在于人类中; elevently看似无害的感染;不表现出季节性波动或流行高峰;主要通过粪-口途径传播;并且可以使用快速分子技术进行检测。这项研究将分四个阶段进行：（1）开发一种可靠的TT病毒检测方法，（2）监测TT病毒在水源和水源沃茨中的密度和发生情况，（3）评估TT病毒与细菌指示物、雄性特异性大肠杆菌噬菌体、诺如病毒和腺病毒的共存情况，以及（4）将TT病毒数据与大肠菌群、大肠杆菌和肠道病毒的发生和存活数据进行比较。该项目将通过评估一个尚未测试的指标，大大扩展对病毒病原体风险的理解和TT病毒作为指标系统的效用，并将有可能开发一个全新的、可靠的、有效的病毒病原体风险指标系统。该项目将包括对学生的支持，并将为每个人提供多学科的经验。学生将有机会在会议上展示成果。调查人员是女性，以前的工作中涉及的学生人数不足。预计该项目将有助于改善公共卫生，其成果将广泛传播。