Differentiation Engineering for Beneficial Alteration of Cellular Traits

细胞性状有益改变的分化工程

• 批准号: 0853490
• 负责人: Eleftherios Papoutsakis
• 金额: $ 45万
• 依托单位: University of Delaware
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0853490&HistoricalAwards=false
• 关键词: Differentiation; Engineering; Beneficial; Alteration; Cellular

0853490PapoutsakisIntellectual Merit - This project aims to develop novel approaches for generating industrially important strains for the production of chemicals and other metabolites, including biofuels. The concept is termed Differentiation Engineering, which is defined here based on its goals: to probe and understand the underlying signal-transduction of differentiation thus enabling genetic or other alterations of cellular differentiation in order to achieve desirable cellular phenotypes. Broader Impacts - The Differentiation Engineering approaches described in the proposal can be applied to other clostridia and other differentiating organisms. In addition, the knowledge generated from this project will make a significant contribution to basic biology, differentiation and genomics of all clostridia and of other sporulating, but not well-understood, organisms. Finally, this project provides unique opportunities for student education and training in an interdisciplinary environment on genetic engineering for strain development, prokaryotic flow cytometry, experimental and computational genomics, and bioreaction engineering.

0853490 Papoutsakis智力优点-该项目旨在开发新的方法，以产生工业上重要的菌株，用于生产化学品和其他代谢产物，包括生物燃料。这一概念被称为分化工程，其定义基于其目标：探索和理解分化的潜在信号转导，从而使细胞分化的遗传或其他改变成为可能，以实现所需的细胞表型。更广泛的影响-提案中描述的分化工程方法可以应用于其他梭菌和其他分化生物。此外，从这个项目产生的知识将作出重大贡献的基础生物学，分化和基因组学的所有梭菌和其他孢子形成，但不太清楚，有机体。最后，该项目为学生提供了独特的机会，在跨学科的环境中对菌株开发，原核流式细胞术，实验和计算基因组学以及生物反应工程的基因工程进行教育和培训。