Differentiation Engineering for Beneficial Alteration of Cellular Traits

[853490] papoutsakisintellectual Merit -该项目旨在开发新的方法，以产生工业上重要的菌株，用于生产化学品和其他代谢物，包括生物燃料。这个概念被称为分化工程，其定义基于其目标：探测和理解分化的潜在信号转导，从而使细胞分化的遗传或其他改变成为可能，以实现理想的细胞表型。更广泛的影响-提案中描述的分化工程方法可以应用于其他梭菌和其他分化生物。此外，该项目所产生的知识将对所有梭状芽孢杆菌和其他孢子生物的基础生物学、分化和基因组学做出重大贡献。最后，该项目为学生提供了独特的机会，在跨学科的环境中进行菌株开发的基因工程，原核流式细胞术，实验和计算基因组学以及生物反应工程的教育和培训。

0853490PapoutsakisIntellectual Merit - This project aims to develop novel approaches for generating industrially important strains for the production of chemicals and other metabolites, including biofuels. The concept is termed Differentiation Engineering, which is defined here based on its goals: to probe and understand the underlying signal-transduction of differentiation thus enabling genetic or other alterations of cellular differentiation in order to achieve desirable cellular phenotypes. Broader Impacts - The Differentiation Engineering approaches described in the proposal can be applied to other clostridia and other differentiating organisms. In addition, the knowledge generated from this project will make a significant contribution to basic biology, differentiation and genomics of all clostridia and of other sporulating, but not well-understood, organisms. Finally, this project provides unique opportunities for student education and training in an interdisciplinary environment on genetic engineering for strain development, prokaryotic flow cytometry, experimental and computational genomics, and bioreaction engineering.